Enteroids for Nutritional Studies. Issue 16 (10th April 2019)

• Record Type: Journal Article
• Authors: Yin, Yue‐Bang; de Jonge, Hugo R.; Wu, Xin; Yin, Yu‐Long
• Is Part Of: Molecular nutrition & food research. Volume 63:Issue 16(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-04-10
• Subjects: enteroid models -- intestinal barriers -- intestinal stem cells -- location‐specific functions -- nutrient transport; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201801143
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract: Nutritional studies are greatly hampered by a paucity of proper models. Previous studies on nutrition have employed conventional cell lines and animal models to gain a better understanding of the field. These models lack certain correlations with human physiological responses, which impede their applications in this field. Enteroids are cultured from intestinal stem cells and include enterocytes, enteroendocrine cells, goblet cells, Paneth cells, and stem cells, which mimic hallmarks of in vivo epithelium and support long‐term culture without genetic or physiological changes. Enteroids have been used as models to study the effects of diet and nutrients on intestinal growth and development, ion and nutrient transport, secretory and absorption functions, the intestinal barrier, and location‐specific functions of the intestine. In this review, the existing models for nutritional studies are discussed and the importance of enteroids as a new model for nutritional studies is highlighted. Taken together, it is suggested that enteroids can serve as a potential model system to be exploited in nutritional studies. Abstract : Intestinal stem cells are settled at the bottom of intestinal crypts, which can grow into enteroids via supplementation with a variety of stemness supporters. Enteroids are characterized as two formats: either as three‐dimensional or two‐dimensional. Both formats of enteroids consist of various intestinal cell types. Enteroids are proper in vitro models for nutritional studies.