Analysis of in vitro digestion using human gut microbiota in adult and elderly individuals. (15th November 2021)

• Record Type: Journal Article
• Authors: Lee, Seung Yun; Lee, Da Young; Kang, Hea Jin; Kang, Ji Hyeop; Jang, Hae Won; Kim, Bum Keun; Hur, Sun Jin
• Is Part Of: Food chemistry. Volume 362(2021)
• Publication Date: 2021-11-15
• Subjects: In vitro human digestion -- Gut microbiota -- Simulating digestion phases -- Aging -- Digestibility -- Bioactive materials; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.130228
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: In vitro digestion models reflected digestion characteristics in adults and elderly. E. coli and Lactobacillus between adult and elderly were to be contrastively at a ratio of 2:3. Digestibility of bioactive materials was decreased in digestion condition of elderly. This protocol can be applied to analysis of food or drug delivery depending on age. Abstract: In vitro human digestion models are widely used to determine the digestibility of bioactive substances and to perform drug delivery analyses. To develop the most accurate in vitro human digestion model reported to date, we simulated all digestion conditions, including pH and digestion time, with changes in the amount of digestive enzymes, motility, and proportion of human gut microbiota in adult and elderly individuals. Using this newly developed model, the digestibility of vitamin E emulsified by lard was found to be significantly different between adults and the elderly. Therefore, this model can accurately simulate oral, gastric, and intestinal (with gut microbiota effects) digestion of bioactive substances and can aid in analyzing drug delivery in adults and elderly individuals.