Determination of 8 biogenic amines in aquatic products and their derived products by high-performance liquid chromatography-tandem mass spectrometry without derivatization. (1st November 2021)

• Record Type: Journal Article
• Authors: Zhang, Xuan; Fang, Changling; Huang, Dongmei; Yang, Guangxin; Tang, Yunyu; Shi, Yongfu; Kong, Cong; Cao, Pei; Cai, Youqiong
• Is Part Of: Food chemistry. Volume 361(2021)
• Publication Date: 2021-11-01
• Subjects: HPLC-MS/MS high-performance liquid chromatography-tandem mass spectrometry -- SPD spermidine -- SPM spermine -- CAD cadaverine -- HIS histamine -- PUT putrescine -- TYR tyramine -- 2-PHE 2-phenylethylamine -- TRY tryptamine -- R2 coefficient of determination -- RSD relative standard deviation -- BA biogenic amine -- FDA Food and Drug Administration -- EU European Union -- EFSA European Food Safety Agency -- WHO/FAO World Health Organization/Food and Agriculture Organization of the United Nat -- JECFA Joint FAO/WHO Expert Committee on Food Additives -- ARfD Acute Reference Dose -- CE capillary electrophoresis -- TLC thin layer chromatography -- GC–MS gas chromatography-mass spectrometry -- HPLC high performance liquid chromatography -- UPLC ultra high performance liquid chromatography -- MRM multiple reaction monitoring -- LOD limit of detection -- LOQ limit of quantification -- HClO perchloric acid -- TCA trichloroacetic acid -- NaOH sodium hydroxide -- MCX oasis mixed cation exchange -- SPE solid-phase extraction -- PTFE polytetrafluoroethylene -- LLE liquid-liquid extraction -- LPME liquid-phase microextraction -- HS-SPME head-space solid-phase microextraction -- NYL nylon membrane filters -- PES polyethersulfone membrane filters; High-performance liquid chromatography-tandem mass spectrometry -- Biogenic amines -- Aquatic products; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.130044
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: HPLC-MS/MS was used for 8 BAs detection in aquatic products and their derived products. 5% perchloric acid solution and n -hexane were selected for extraction and purification. The pretreatment was rapid and simple without derivatization. A wide linear range, good sensitivity and accuracy were obtained. Abstract: A method for the determination of 8 biogenic amines in aquatic products and their derived products was established by HPLC-MS/MS without derivatization. The samples were extracted by 5% perchloric acid solution. N -hexane was used to clean the extract. The analytes were separated by a column of ACQUITY UPLC HSS T3 (100 mm × 2.1 mm, 1.8 µm), and gradient eluted with a mixed solution of (0.5% formic acid) and acetonitrile. Good linearity was obtained with correlation coefficients (R 2 ) >0.99. This method achieved higher sensitivity (from 0.1 mg/kg for tyramine, 2-phenylethylamine and tryptamine to 1.0 mg/kg for spermidine, spermine, cadaverin, histamine and putrescine). The average recoveries were demonstrated in the range of 70.9%–113.1%, with relative standard deviations (RSDs) from 0.33% to 10.81%. This method was suitable for the detection of BAs in aquatic products and their products.