Nanoparticles derived from porcine bone soup attenuate oxidative stress-induced intestinal barrier injury in Caco-2 cell monolayer model. (August 2021)

• Record Type: Journal Article
• Authors: Gao, Guanzhen; Zhou, Jianwu; Jin, Yongyang; Wang, Huiqin; Ding, Yanan; Zhou, Jingru; Ke, Lijing; Rao, Pingfan; Chong, Pik Han; Wang, Qiang; Zhang, Longxin
• Is Part Of: Journal of functional foods. Volume 83(2021)
• Publication Date: 2021-08
• Subjects: Bone soup -- Nanoparticles -- Oxidative stress -- Intestinal barrier injury -- Caco-2 cell monolayer -- AAPH; NPs Nanoparticles -- ENMS Engineered nanomaterials -- GI Gastrointestinal -- TEER Transepithelial/transendothelial electrical resistance -- TJs Tight junctions -- AJs Adherent junctions -- AAPH 1, 1-Diphenyl-2-picrylhydrazyl radical 2, 2-Diphenyl-1-(2, 4, 6-trinitrophenyl) hydrazyl -- FL Sodium fluorescein -- CI Cell Index -- SEC-DLS Size-exclusion chromatography coupled with a dynamic light scattering detector -- TEM Transmission electron microscope -- IBD Inflammatory bowel disease -- UC Ulcerative colitis -- IBS Irritable bowel syndrome; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2021.104573
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: A large number of nanoparticles occurred in porcine bone soup. Their interaction with intestinal cells should be noted, but is barely studied yet. Those particles restored intestinal barrier dysfunction caused by oxidative stress. They restored oxidative stress caused damages on TJs and AJs related proteins. Abstract: Safety concerns arose on the interaction between nanoparticles in food and intestinal tract. Food components could spontaneously assemble into a large number of nanoparticles during food processing. These nanoparticles may possess physiological effects differed from those of constituent components, are worth paying attention to, but are barely investigated yet, especially on their interaction with intestinal tract. Porcine bone soup is rich in nanoparticles, which can directly interact with oral macrophages disclosed by our previous study. In this study, the effects of bone soup nanoparticles on intestinal barrier function were subsequently evaluated on Caco-2 cell monolayers. The results revealed the nanoparticles did not develop but restore intestinal barrier dysfunction compared with engineered nanoparticles, indicated by barrier integrity, sodium fluorescein permeability, tight junctions and adherent junctions related proteins. These results showed the potential of bone soup nanoparticles on improving intestinal disorders, which resonated with traditional knowledge on the efficacies of bone soup.