Rapid biofilm eradication of the antimicrobial peptide 1018-K6 against Staphylococcus aureus: A new potential tool to fight bacterial biofilms. (January 2020)

• Record Type: Journal Article
• Authors: Colagiorgi, Angelo; Festa, Rossella; Di Ciccio, Pierluigi A.; Gogliettino, Marta; Balestrieri, Marco; Palmieri, Gianna; Anastasio, Aniello; Ianieri, Adriana
• Is Part Of: Food control. Volume 107(2020)
• Publication Date: 2020-01
• Subjects: Biofilms -- Foodborne pathogens -- Staphylococcus aureus -- Antimicrobial peptides -- Food safety; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2019.106815
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Staphylococcal food poisoning – caused by certain enterotoxigenic staphylococci – is one of the most common worldwide foodborne diseases. In this context, bacterial biofilms on food processing plan surfaces can represent critical sources of contamination, being more resistant to cleaning and disinfection procedures. Therefore, it is important to prevent and control biofilm formation in food facilities. In the last years, antimicrobial peptides (AMPs) have emerged as promising biofilm contrasting tools. The recently developed AMP, named 1018-K6, revealed a significant bactericidal (MBC 10 μM) and biofilm-preventing efficiency (MBIC 50 μM) against a reference ATCC strain of Listeria monocytogenes and remarkable stability under different environmental conditions. In this work, a kinetic of action of 1018-K6 against two strong biofilm-producing reference strains of Staphylococcus aureus (including a methicillin-resistant S. aureus strain) and a moderate biofilm producer enterotoxigenic S. aureus isolated from cheese, was performed. The peptide showed an impressive rapid mode of action, eradicating established biofilms within few minutes. Bactericidal activity against planktonic cells and inhibition of biofilm formation were also observed. The significant properties of 1018-K6 make it a promising candidate for applications in food-safety and quality control. Highlights: 1018-K6 shows a strong and rapid anti-biofilm action against S. aureus strains. MRSA strain is highly sensible to the antimicrobial action of 1018-K6. Biofilm of food-isolated SA1 strain was completely eradicated by 1018-K6. Cell membrane-affecting mode of action of 1018-K6. A new peptide-based solution for applications in food safety and quality control.