The groESL ISR sequence-based species-specific identification of GRAS and non-GRAS Lactiplantibacillus as an alternative to 16S rRNA sequencing. (July 2021)

• Record Type: Journal Article
• Authors: Lee, Jong Min; Park, So Hee; Jin, Chun-Zhi; Kang, Min-Kyoung; Park, Dong-Jin; Kim, Chang-Jin
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 147(2021)
• Publication Date: 2021-07
• Subjects: Identification -- Lactiplantibacillus -- Lactobacillus -- Intergenic spacer region -- groESL; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2021.111504
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: The untranscribed DNA sequences of the intergenic spacer regions (ISR) in the groESL were analyzed to resolve the ambiguity within phylogenetically close GRAS and non-GRAS species. The sequencing results of amplified polymerase chain reaction (PCR) products using groESL-ISR-specific primers accurately distinguished L . plantarum and L . pentosus, which were not distinguished by 16 S rRNA sequences. Furthermore, the 20 selected major probiotics species were divided into several groups according to the length (22–91 bp) and homology (65–99%) of their ISR sequences, and this discrimination was consistent with core-genome-based differentiation. Therefore, ISR sequence-based species identification showed more accurate results than 16 S rRNA sequence-based identification and represented genome-based speciation. Highlights: L. plantarum and L. pentosus cannot be distinguished by the 16 S rRNA sequence. The groESL operon sequences are species-specific. The groESL operon sequences represented whole-genome-based taxonomy. The groESL ISRs are proposed as a target sequence alternative to 16 S rRNA sequencing. In this report, ISR sequences of probiotics and universal primers for PCR amplification are provided.