Host defense peptides identified in human apolipoprotein B as novel food biopreservatives and active coating components. (October 2021)

• Record Type: Journal Article
• Authors: Dell'Olmo, Eliana; Gaglione, Rosa; Sabbah, Mohammed; Schibeci, Martina; Cesaro, Angela; Di Girolamo, Rocco; Porta, Raffaele; Arciello, Angela
• Is Part Of: Food microbiology. Volume 99(2021)
• Publication Date: 2021-10
• Subjects: Active coating -- Antibiofilm peptides -- Antimicrobial peptides -- Chitosan -- Food biopreservatives -- Host defence peptides; ApoB apolipoprotein B -- CH chitosan -- WHO World Health Organization -- HDPs host defence peptides -- MHB Muller Hinton Broth -- TSA Tryptic Soy Agar -- NB Nutrient Broth -- MBC minimal bactericidal concentration -- SEM scanning electron microscopy -- ETD Everhart Thornley Detector -- PCA Plate count agar -- HSD Honestly Significant Difference; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2021.103804
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: The effectiveness of three novel "host defence peptides" identified in human Apolipoprotein B (ApoB) as novel antimicrobial and antibiofilm agents to be employed in food industry is reported. ApoB-derived peptides have been found to exert significant antimicrobial effects towards Salmonella typhimurium ATCC® 14028 and Salmonella enteritidis 706 RIVM strains. Furthermore, they have been found to retain antimicrobial activity under experimental conditions selected to simulate those occurring during food storage, transportation and heat treatment, and have been found to be endowed with antibiofilm properties. Based on these findings, to evaluate the applicability of ApoB-derived peptides as food biopreservatives, coating solutions composed by chitosan (CH) and an ApoB-derived peptide have been prepared and found to be able to prevent Salmonella cells attachment to different kinds of surfaces employed in food industry. Finally, obtained coating solution has been demonstrated to hinder microbial proliferation in chicken meat samples. Altogether, obtained findings indicate that ApoB-derived peptides are promising candidates as novel biopreservatives for food packaging. Graphical abstract: Image 1 Highlights: ApoB-derived peptides exert antimicrobial and antibiofilm effects on Salmonella spp. A novel CH coating solution containing ApoB-derived peptides has been prepared. Bioactive coating solution prevents Salmonella attachment to industrial surfaces. Chicken meat coating with prepared solution hinders food spoilage during storage.