Applicative Insights on Nascent Role of Biochar Production, Tailoring and Immobilization in Enzyme Industry -A Review. (August 2021)

• Record Type: Journal Article
• Authors: Kaur, Naviljyot; Bhardwaj, Priyanka; Singh, Gursharan; Arya, Shailendra Kumar
• Is Part Of: Process biochemistry. Volume 107(2021)
• Page Start: 153
• Page End: 163
• Publication Date: 2021-08
• Subjects: Biochar -- Enzyme immobilization -- Contaminant removal -- Antibiotic degradation; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2021.05.017
• Languages: English
• ISSNs: 1359-5113

Abstract

Highlights: Biochar has versatile characteristic for enzyme immobilization support. Immobilization aids to increased shelf life, stability and reusability of enzyme. Adsorption aids to be profound economic method preventing enzyme conformational alteration. Covalent binding averts enzyme leeching and desorption. Enzyme biochar complex is an efficient contaminant removal system and has potential to increase enzyme activity in bio-fuel production. Abstract: Biochar or agrichar the carbon rich source has been appreciated globally for its versatile properties like large surface area, high level porosity, thermal stability and long shelf life. The advent of such properties and the fact that it can be obtained at low operational costs has created phenomenal applications for biochar to be used in biotechnological and agricultural industries. Availability and high cost of production of enzymes has lead immobilization to be served as fruitful technique to maintain the price efficacy. Endowing to its properties like surface availability of functional groups, being an exceptional biosorbent it is availed as potential carrier for immobilization. For better applicability, various physio-chemical tailoring strategies have been adopted to perk up the usage. The main mechanism that works for biochar immobilization is adsorption due to high surface porosity, surface area of biochar. Further aiding to perks of adsorption being simple, cost effective and economical approach that maintains structural integrity of the enzymes. In addition to this covalent binding can vanquish the demerit enzyme leeching caused by adsorption.This review centers on various methods for production of biochar, a range of tailoring mechanisms being followed, and applicative usages of biochar immobilized enzymes in diverse processes.