Assessment of the biological activity of fish muscle protein hydrolysates using in vitro model systems. (15th October 2021)

• Record Type: Journal Article
• Authors: Heffernan, Shauna; Giblin, Linda; O'Brien, Nora
• Is Part Of: Food chemistry. Volume 359(2021)
• Publication Date: 2021-10-15
• Subjects: AA amino acid -- AAPH 2, 2-azobis-(2-amidino- propane) dihydrochloride -- ABTS 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid -- ACE angiotensin-1-converting enzyme -- BMI body mass index -- BWPH blue whiting protein hydrolysate -- CAT catalase -- CCK cholecystokinin -- C/EBPα CCATT/enhancer binding protein α -- CFU colony forming unit -- COX-2 cyclooxygenase-2 -- DH degree of hydrolysis -- DPPH 2, 2-diphenyl-1- picrylhydrazyl -- EC50 concentration corresponding to half-maximal activity -- E/S ratio enzyme/ substrate ratio, FPH, fish protein hydrolysates -- FOSHU Foods for Specific Health Use -- FRAP ferric reducing antioxidant power -- GCB graphitized carbon black -- GI gastrointestinal -- GLP-1 glucagon-like peptide-1 -- GSH glutathione -- GSH-Px glutathione peroxidase -- GPH goby protein hydrolysate -- H2O2 hydrogen peroxide -- IL interleukin -- LPH lanternfish protein hydrolysates -- LPS lipopolysaccharide -- iNOS inducible nitric oxide synthase -- MAPK mitogen-activated protein kinase -- MIC minimum inhibition concentration -- MW molecular weight -- NF nuclear factor -- NO nitric oxide -- O2– superoxide anion -- ORAC oxygen radical absorbance capacity -- OH hydroxyl -- PPARγ peroxisome proliferator-activated receptor γ -- PGE2 prostaglandin E2 -- PYY peptide YY -- RPH ray protein hydrolysate -- ROS reactive oxygen species -- RP-HPLC reversed-phase high-performance liquid chromatography -- RSM response surface methodology -- SGID simulated gastrointestinal digestion -- SOD superoxide dismutase -- SPH sardinella protein hydrolysate -- SREBP-1 sterol regulatory element binding protein -- TNF-α tumour necrosis factor-α -- ZPH zebra blenny protein hydrolysate; Fish protein hydrolysates -- Enzymatic hydrolysis -- In vitro -- Bioactivity; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.129852
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Processing conditions have major effects on bioactivity of fish protein hydrolysates. Low molecular weight fish hydrolysates generally exhibit enhanced bioactivities. Identification of bioactive peptide(s) from fish protein hydrolysates is limited. Animal and human studies on fish muscle hydrolysates/peptides are required. Abstract: The generation of biologically active fish protein hydrolysates (FPH) is a useful technique to produce value-added products with potential application in the functional food and nutraceutical industries. Fish muscle is an attractive substrate for the production of protein hydrolysates due to its rich protein content, containing 15–25% of total fish protein. This paper reviews the production of protein hydrolysates from fish muscle, most commonly via enzymatic hydrolysis, and their subsequent bioactivities including anti-obesity, immunomodulatory, antioxidant, angiotensin I‐converting enzyme (ACE)-inhibitory, anti-microbial, and anti-cancer activities as measured by in vitro testing methods. Disease prevention with FPH potentially offers a safe and natural alternative to synthetic drugs. Small molecular weight (MW) FPHs generally exhibit favourable bioactivity than large MW fractions via enhanced absorption through the gastrointestinal tract. This review also discusses the relationship between amino acid (AA) composition and AA sequence of FPH and peptides and their exhibited in vitro bioactivity.