Asymmetrical flow field-flow fractionation combined with electrophoresis: A new approach for studying thermal aggregation behavior of soy protein isolate. (October 2021)

• Record Type: Journal Article
• Authors: Wang, Wei; Zhang, Juhua; Zhang, Xirui; Guo, Yuxi; Shi, Jianhong; Shen, Shigang; Han, Dandan; Dou, Haiyang
• Is Part Of: Food hydrocolloids. Volume 119(2021)
• Publication Date: 2021-10
• Subjects: Soy protein isolate -- Aggregation -- Asymmetrical flow field-flow fractionation -- SDS-PAGE; Hydrocolloids -- Periodicals; Food additives -- Periodicals; Colloïdes -- Périodiques; Aliments -- Additifs -- Périodiques; Colloids; Food additives; Periodicals; Electronic journals; 664.06
• DOI: 10.1016/j.foodhyd.2021.106857
• Languages: English
• ISSNs: 0268-005X

Abstract

Abstract: The thermal aggregation behavior of soy protein isolate (SPI) has a significant impact on the functional and nutritional properties of soy protein based-food. In this study, the capability of asymmetrical flow field-flow fractionation (AF4) combined with sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) for monitoring the thermal aggregation behavior of SPIs in situ was evaluated. The effects of heating temperature and time, protein concentration, ionic strength and type of salt on the thermal aggregation behavior of SPIs were investigated. Two types of SPIs subunits aggregates with different sizes and compositions in this work were observed, which mainly owes to the utilization of open channel in AF4 as well as SDS-PAGE. Furthermore, the results suggested that larger SPIs aggregates can dissociate, rearrange, and aggregate to form the smaller ones at a long heating time. Zeta potential and turbidity results revealed that higher ionic strength promotes the formation of larger SPIs aggregates. AF4 combined with SDS-PAGE was proved to be a powerful tool for studying the thermal aggregation behavior of SPIs. Highlights: The thermal aggregation behavior of soy protein isolate (SPIs) was investigated. Asymmetrical flow field-flow fractionation was employed for the separation of SPIs. SDS-PGAE was applied for the analysis of AF4 fraction of SPIs treated by heating. Two types of SPI subunits aggregates were observed under the heat conditions used.