Development of a sensitive polyclonal antibody-based competitive indirect ELISA for determination of citrinin in grain-based foods. Issue 10 (3rd October 2019)

• Record Type: Journal Article
• Authors: Singh, Gurmit; Velasquez, Ligia; Huet, Anne-Catherine; Delahaut, Philippe; Gillard, Nathalie; Koerner, Terry
• Is Part Of: Food additives & contaminants. Volume 36:Issue 10(2019)
• Page Start: 1567
• Page End: 1573
• Publication Date: 2019-10-03
• Subjects: Citrinin -- polyclonal antibody -- ciELISA -- z-score -- grain-based foods; Food additives -- Periodicals; Food contamination -- Periodicals; 664.06
• DOI: 10.1080/19440049.2019.1640895
• Languages: English
• ISSNs: 1944-0049

Abstract

ABSTRACT: A sensitive competitive indirect enzyme-linked immunosorbent assay (ciELISA) was developed for the detection and quantification of citrinin (CIT) in grain-based food samples. The limit of quantification (IC20 ) of the established method was 0.10 ± 0.02 ng mL −1, with the limit of detection (IC10 ) being 0.04 ± 0.007 ng mL −1 in wheat and corn flour matrices with a coefficient of variation (CV) less than 20%. The assay was very specific to CIT and showed no cross-reactivity with other mycotoxins (OTA, T-2 toxin, HT-2 toxin, DON, patulin and zearalenone). In spiked wheat and corn flours, the recoveries ranged from 86.6% to 115.6% with CVs of less than 20%. The effectiveness of this method was verified by participating in a proficiency test (PT) from the Food Analysis Performance Assessment Scheme (FAPAS) 17181 corn flour. A successful z-score (−0.6) for this PT sample showed that the present method is comparable to the instrumental methods used by other laboratories in the PT testing scheme. A small survey of grain-based foods was conducted using this method and CIT was detected in 43% of the samples up to a concentration of 17.7 ng g −1 . This method is suitable for sensitive and rapid quantitation of citrinin in wheat and corn matrices.