Dietary Spray-Dried Porcine Plasma Prevents Cognitive Decline in Senescent Mice and Reduces Neuroinflammation and Oxidative Stress. Issue 2 (3rd October 2019)

• Record Type: Journal Article
• Authors: Garcia-Just, Alba; Miró, Lluïsa; Pérez-Bosque, Anna; Amat, Concepció; Polo, Javier; Pallàs, Mercè; Griñán-Ferré, Christian; Moretó, Miquel
• Is Part Of: Journal of nutrition. Volume 150:Issue 2(2020)
• Page Start: 303
• Page End: 311
• Publication Date: 2019-10-03
• Subjects: neuroinflammation -- cognitive decline -- aging -- spray-dried animal plasma -- dietary supplementation; Nutrition -- Periodicals; Diet -- Periodicals; 613.205
• DOI: 10.1093/jn/nxz239
• Languages: English
• ISSNs: 0022-3166

Abstract

ABSTRACT: Background: Aging is characterized by chronic, low-grade inflammation that correlates with cognitive decline. Dietary supplementation with spray-dried porcine plasma (SDP) reduces immune activation in rodent models of inflammation and aging. Objective: We investigated whether the anti-inflammatory properties of SDP could ameliorate age-related cognitive deterioration and preserve brain homeostasis in an aging mouse model of senescence. Methods: Male senescence-accelerated prone 8 (SAMP8) mice were used. In Experiment 1, cognitive performance ( n = 10–14 mice/group) was analyzed by the novel object recognition test in 2-mo-old mice (2M group) and in mice fed a control diet or a diet supplemented with 8% SDP for 2 (4M-CTL and 4M-SDP groups) and 4 mo (6M-CTL and 6M-SDP groups). In Experiment 2, the permeability of the blood–brain barrier and junctional proteins in brain tissue was assessed, as well as synaptic density, oxidative stress markers, and inflammatory genes and proteins in mice from the 2M, 6M-CTL, and 6M-SDP groups ( n = 5–11). Statistical analyses included one-factor ANOVA followed by Fisher's posthoc test. Results: 6M-SDP mice had better cognitive performance than 6M-CTL mice in both short-term ( P = 0.024) and long-term ( P = 0.017) memory tests. In brain tissue, 6M-SDP mice showed reduced brain capillary permeability ( P = 0.034) and increased ZO1 and E-cadherin expression (both P <0.04) compared with 6M-CTL mice. SDP also prevented the NFκB activation observed in 6M-CTL mice ( P = 0.002) and reduced Il6 expression and hydrogen peroxide concentration (both P <0.03) observed in 6M-CTL mice. SDP also increased the concentration of IL10 ( P = 0.027), an anti-inflammatory cytokine correlated with memory preservation. Conclusions: In senescent SAMP8 mice, dietary supplementation with SDP attenuated cognitive decline and prevented changes in brain markers of neuroinflammation and oxidative stress.