GNPS-guided discovery of xylacremolide C and D, evaluation of their putative biosynthetic origin and bioactivity studies of xylacremolide A and B. Issue 31 (24th May 2021)

• Record Type: Journal Article
• Authors: Schalk, Felix; Fricke, Janis; Um, Soohyun; Conlon, Benjamin H.; Maus, Hannah; Jäger, Nils; Heinzel, Thorsten; Schirmeister, Tanja; Poulsen, Michael; Beemelmanns, Christine
• Is Part Of: RSC advances. Volume 11:Issue 31(2021)
• Page Start: 18748
• Page End: 18756
• Publication Date: 2021-05-24
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1ra00997d
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Targeted HRMS 2 -GNPS-based metabolomic and genomic analysis of the fungal garden antagonist Pseudoxylaria sp. X187 resulted in the identification of xylacremolide C and D and the identification of their putative PKS-NRPS-based biosynthesis. Abstract : Targeted HRMS 2 -GNPS-based metabolomic analysis of Pseudoxylaria sp. X187, a fungal antagonist of the fungus-growing termite symbiosis, resulted in the identification of two lipopeptidic congeners of xylacremolides, named xylacremolide C and D, which are built from d -phenylalanine, l -proline and an acetyl-CoA starter unit elongated by four malonyl-CoA derived ketide units. The putative xya gene cluster was identified from a draft genome generated by Illumina and PacBio sequencing and RNAseq studies. Biological activities of xylacremolide A and B were evaluated and revealed weak histone deacetylase inhibitory (HDACi) and antifungal activities, as well as moderate protease inhibition activity across a panel of nine human, viral and bacterial proteases.