GNPS-guided discovery of xylacremolide C and D, evaluation of their putative biosynthetic origin and bioactivity studies of xylacremolide A and B. Issue 31 (24th May 2021) - Schalk, Felix
Abstract : Targeted HRMS 2 -GNPS-based metabolomic and genomic analysis of the fungal garden antagonist Pseudoxylaria sp. X187 resulted in the identification of xylacremolide C and D and the identification of their putative PKS-NRPS-based biosynthesis. Abstract : Targeted HRMS 2 -GNPS-based metabolomic analysis of Pseudoxylaria sp. X187, a fungal antagonist of the fungus-growing termite symbiosis, resulted in the identification of two lipopeptidic congeners of xylacremolides, named xylacremolide C and D, which are built from d -phenylalanine, l -proline and an acetyl-CoA starter unit elongated by four malonyl-CoA derived ketide units. The putative xya gene cluster was identified from a draft genome generated by Illumina and PacBio sequencing and RNAseq studies. Biological activities of xylacremolide A and B were evaluated and revealed weak histone deacetylase inhibitory (HDACi) and antifungal activities, as well as moderate protease inhibition activity across a panel of nine human, viral and bacterial proteases.