Comparison of two kinds of peroxyl radical pretreatment at chicken myofibrillar proteins glycation on the formation of Nε-carboxymethyllysine and Nε-carboxyethyllysine. (15th August 2021)

• Record Type: Journal Article
• Authors: Zhu, Zongshuai; Yang, Jing; Zhou, Xinghu; Khan, Iftikhar Ali; Bassey, Anthony Pius; Huang, Ming
• Is Part Of: Food chemistry. Volume 353(2021)
• Publication Date: 2021-08-15
• Subjects: Peroxyl radical -- Glycosylation -- Myofibrillar proteins -- Nε-carboxymethyllysine -- Nε-carboxyethyllysine; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.129487
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Impacts of CML and CEL formation induced by ROO· and LOO· were compared. Effects of peroxyl radical on protein gel microstructure were investigated. Peroxyl radical changed the protein oxidation and Maillard reaction. Protein oxidation and glycation promoted CML and CEL formation by altering structure modification of protein gel. Abstract: During meat processing, two typical advanced glycation end products (AGEs), N ε -carboxymethyllysine (CML) and N ε -carboxyethyllysine (CEL), are generated by free radical induction. However, the impact of peroxyl radicals on myofibrillar proteins (MPs) glycosylation and CML and CEL formation is scarcely reported. In this study, two peroxyl radicals called ROO· and LOO· derived from AAPH (2, 2′-azobis (2-methylpropionamidine) dihydrochloride) and linoleic acid were exposed prior to the Maillard reaction (glucosamine incubation at 37 °C for 24 h). Levels of AGEs (CML/CEL), protein oxidation (sulfhydryl/carbonyl), free amino group, surface hydrophobicity, zeta potential, particle size, intrinsic fluorescence intensity and secondary structure were determined. Together with Pearson's correlation, the assumption that free radicals promote MPs oxidation and glycation, alter the aggregation behavior and structure modification, leading to AGEs promotion has been built. In addition, the effect of dose-dependency of peroxyl radical on AGEs has also been established with different effects of peroxyl radical induction.