Type I collagen from sea cucumber (Stichopus japonicus) and the role of matrix metalloproteinase-2 in autolysis. (June 2021)

• Record Type: Journal Article
• Authors: Yan, Long-Jie; Sun, Le-Chang; Cao, Kai-Yuan; Chen, Yu-Lei; Zhang, Ling-Jing; Liu, Guang-Ming; Jin, Tengchuan; Cao, Min-Jie
• Is Part Of: Food bioscience. Volume 41(2021)
• Publication Date: 2021-06
• Subjects: Stichopus japonicas -- Sea cucumber autolysis -- Collagen -- Molecular cloning -- Matrix metalloproteinase-2; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2021.100959
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: Autolysis is a major issue affecting the quality of sea cucumber during transportation and processing. To further clarify the mechanism, characteristics of type I collagen from sea cucumber Stichopus japonicas and its degradation by recombinant matrix metalloproteinase-2 (rMMP-2) were investigated. Scanning electron microscopic (SEM) images showed that the body wall of fresh sea cucumber with intact collagen fibrils was disaggregated after autolysis. Pepsin-soluble collagen of Stichopus japonicas ( Sj PSC) was prepared and its filamentous feature was observed using SEM. Circular dichroism spectrum analysis showed that Sj PSC had native triple helix structure and its denaturation temperature was 34.0 °C using differential scanning calorimeter. The α chain gene of Sj PSC encoding 1400 amino acid residues was cloned. A polyclonal antibody against Sj PSC was prepared and was used to detect collagen degradation during autolysis. The effect of rMMP-2 on the body wall strongly suggested that MMP-2 had an important role in sea cucumber autolysis by disaggregating collagen fibres into fibrils. Graphical abstract: Image 1 Highlights: Sj PSC was purified and its morphological features in the solution state were observed. A highly specific polyclonal antibody against Sj PSC was prepared. The α chain gene of Sj PSC was cloned. MMP-2 has an important role in the degradation of collagen fibrils during autolysis.