Metabolic profiling and in vivo hepatoprotective activity of Malpighiaglabra L. leaves. Issue 2 (22nd December 2020)

• Record Type: Journal Article
• Authors: El‐Hawary, Seham S.; El‐Fitiany, Rana Ahmed; Mousa, Ola Mohamed; Salama, Abeer A. A.; El Gedaily, Rania A.
• Is Part Of: Journal of food biochemistry. Volume 45:Issue 2(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-12-22
• Subjects: acerola -- flavonoids -- hepatoprotective -- Malpighiaglabra -- phenolic acids -- Q‐TOF LC/MS/MS; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Biochemistry -- Periodicals; 664.024
• DOI: 10.1111/jfbc.13588
• Languages: English
• ISSNs: 0145-8884

Abstract

Abstract: Although Malpighia glabra Linn. fruits are well studied for their nutritional and medicinal prominence; little attention has been given to the leaves. Our study intends to investigate the leaves metabolic profile using Q‐TOF LC/MS/MS (Quadrupole‐Time‐of‐Flight‐Liquid‐Chromatography‐Mass‐Spectrometry), and to explore their in vivo hepatoprotective activity in rats using CCL4 ‐induced hepatic damage model and silymarin as standard. Fifty metabolites were characterized, belonging to different classes; coumarins (capensine, daphnoretin, and scopoletin), flavonoids (mainly quercetin and apigenin glycosides), phenolic acids (cinnamic acid and quinic acid derivatives) and amino acids (adenosine, homoisoleucine, and phenylalanine).These compounds are detected in the leaves for the first time. The hepatoprotective activity at three doses (200, 400, and 800 mg/kg) was investigated. The dose of 800 mg/Kg showed the highest hepatoprotective effect as it reduced the elevated serum levels of ALT, AST, NO, and TNF‐α liver content by 26, 24, 23, and 42%, respectively, it also remarkably increased the serum level of catalase by 102%. All the tested doses showed higher reduction in serum level of TNF‐α compared to silymarin which suggests their strong anti‐inflammatory potential. M . glabra leaves are revealed to be a rich source of secondary metabolites and proved to possess significant hepatoprotective potential. Practical applications: The performed analyses in this study shows the richness of Malpighia glabra Linn. leaves in a plethora of beneficial and safe phytochemicals which are well‐known to have a pivotal role in protection against different diseases including liver disorders. The carried‐out investigations were done using Q‐TOF LC/MS/MS analysis which is a reliable technique for the determination, characterization and identification of bioactive metabolites; in addition to evaluation of the hepatoprotective effect of the leaves. Therefore, this study may emphasize that Malpighia glabra Linn. leaves may have the same nutritional and medicinal importance as its fruits, and they could be incorporated into pharmaceuticals and foods instead of discarding them. Abstract : Fifty metabolites were characterized in the ethanolic extract of Malpighia glabra L. leaves using Q‐TOF LC/MS/MS analysis for the first time. Furthermore, three doses (200, 400, 800 mg/kg) of the ethanolic extract of Malpighia glabra L. leaves were tested for hepatoprotective activity using CCl4 ‐induced liver damage in rats and silymarin as a standard. Biochemical analyses were conducted on liver serum and tissues, in addition to histopathological and immunohistochemistry examinations.