A novel bacteriocin PE-ZYB1 produced by Pediococcus pentosaceus zy-B isolated from intestine of Mimachlamys nobilis: Purification, identification and its anti-listerial action. (January 2020)

• Record Type: Journal Article
• Authors: Zhang, Ying; Yang, Jingming; Liu, Ying; Wu, Yaqian; Fang, Zhijia; Wang, Yaling; Sun, Lijun; Deng, Qi; Gooneratne, Ravi; Xiao, Lixia
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 118(2020)
• Publication Date: 2020-01
• Subjects: Novel bacteriocin -- Purification -- Action mode -- Listeria monocytogenes -- Pediococcus pentosaceus; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2019.108760
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: A novel bacteriocin, PE-ZYB1, was produced by Pediococcus pentosaceus zy-B that was isolated from the intestine of Mimachlamys nobilis and collected from the South China Sea. The bacteriocin PE-ZYB1 was purified using acetic ether extraction, Sephadex LH-20 column chromatography, sepharose cation exchange chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), in succession. Its molecular mass was determined to be MW 2019.22 Da, and its N-terminal amino acid sequence was DLFNKFNRSMQYGNGVE. The PE-ZYB1 was confirmed as a novel bacteriocin through a homology search, using UniProt BLAST (NO. A0A1Y0VLN8). Bacteriocin PE-ZYB1 was found to have extensive spectrum antimicrobial activity against both Gram-positive and Gram-negative bacteria. Bacteriocin PE-ZYB1 showed resistance to heat, was highly stable over pH 2–7 and was sensitive to trypsin. Increased extracellular electrical conductivity was detected for Listeria. monocytogenes treated by bacteriocin PE-ZYB1. Furthermore, bacteriocin PE-ZYB1 bactericidally destroyed the cell membrane integrity of L. monocytogenes, resulting in cell membrane perforation and permeabilization. These results indicate the potential of the bacteriocin PE-ZYB1 for use as a food bio-preservative. Highlights: A novel bacteriocin PE-ZYB1 was produced by Pediococcus pentosaceus zy-B. The molecular mass of the purified bacteriocin PE-ZYB1 was 2019.22 Da. PE-ZYB1 was confirmed as a novel bacteriocin through a homology search. Heat resistant and possess broad spectrum antimicrobial activity of PE-ZYB1. Bacteriocin PE-ZYB1 destroyed the integrity of cell membrane.