Catch and release of concanavalin A by a mannose-immobilized photoaffinity PEGA resin coupled with a cleavable disulfide linker. Issue 12 (2nd December 2015)

• Record Type: Journal Article
• Authors: Kuramochi, Kouji; Matsushita, Tomohisa; Tsubaki, Kazunori
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 79:Issue 12(2015)
• Page Start: 1946
• Page End: 1953
• Publication Date: 2015-12-02
• Subjects: catch and release -- concanavalin A -- mannose; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2015.1060848
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: A photoaffinity PEGA resin containing mannose as a ligand and disulfide as a cleavable linker was prepared. The resin was crosslinked to concanavalin A, a binding protein of mannose, by UV irradiation, and the protein was subsequently released by cleavage of the disulfide linker. Graphical abstract: : This work reports the design, synthesis and evaluation of an affinity resin to catch and release the target protein of a specific ligand