Development of a physicochemical method to quantify the extracellular liquid volume: Application to the transformation of cassava into gari. (July 2019)

• Record Type: Journal Article
• Authors: Rondet, Eric; Dahdouh, Layal; Escobar, Andrès; Ruiz, Emilie; Cuq, Bernard; Delalonde, Michèle
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 108(2019)
• Page Start: 1
• Page End: 5
• Publication Date: 2019-07
• Subjects: Liquid volume localisation -- Extracellular liquid -- Cassava processing -- poly(4-styrenesulfonate) sodium salt; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2019.03.068
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: In fresh vegetal materials, liquid is mostly located in cells. During processing, this liquid is distributed in the entire vegetal matrix volume including exctracellular volume. According to its location, it will be more or less available for physico-chemical mechanisms that takes place during processing of vegetal matrices. This availability greatly influences the quality of the final products. This work consisted in developing a method for determining the quantity of extracellular liquid in a cell-component food matrix. The method developed is spectrophotometric-based and consists in adding a known mass of polymer (poly(4-styrenesulfonate) sodium salt, PSS) to a rasped vegetal material and defining its concentration in the supernatant separated from the solid phase. The PSS molar mass prevents it from penetrating into plant cells. The PSS concentration measured makes it possible to calculate the extracellular liquid volume. The method validation and its application to rasped and processed (fermentation, pressing) cassava makes it possible to obtain results in agreement with those obtained with other methods. Highlights: A new method for extracellular liquid quantification has been developed and validated. Adding PSS to the extracellular liquid did not generate osmotic flows. The sorption phenomenon of the polymer on the solid surfaces is significant. The extracellular fluid extraction method by centrifugation does not alter PSS concentration. This method applied to cassava pulp gave results coherent to that obtained with other methods.