Prevalence, antimicrobial resistance and virulence genes characterization of nontyphoidal Salmonella isolated from retail chicken meat shops in Northern India. (August 2019)

• Record Type: Journal Article
• Authors: Sharma, Jaishree; Kumar, Deepak; Hussain, Sheeba; Pathak, Anubha; Shukla, Maansi; Prasanna Kumar, V.; Anisha, P.N.; Rautela, Richa; Upadhyay, A.K.; Singh, S.P.
• Is Part Of: Food control. Volume 102(2019)
• Page Start: 104
• Page End: 111
• Publication Date: 2019-08
• Subjects: Chicken -- S. Kentucky -- Antibiotic resistance -- Ciprofloxacin -- Virulence; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2019.01.021
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: The objective of this study was to determine the prevalence of antibiotic resistant nontyphoidal Salmonella in 39 retail chicken meat shops located in 7 towns of North India. Overall, nontyphoidal Salmonella prevalence of 9.43% (70/742) was observed. Highest Salmonella prevalence was observed in Lalkuan (20.99%). Chicken meat samples (40%) showed highest Salmonella prevalence followed by poultry feces (20%). Three serotypes of Salmonella were identified, Salmonella Kentucky ( S .Kentucky, 74.29%), Salmonella Virchow ( S .Virchow, 17.14%) and Salmonella Typhimurium ( S .Typhimurium, 7.14%). All isolates were multidrug resistant (MDR) by disk-diffusion assay, showing resistance to three or more classes of antibiotics. High resistance was observed to tetracycline (100%), erythromycin (100%), nalidixic acid (98.57%), ampicillin (95.71%) and ciprofloxacin (82.86%). Fifty-one (98.08%) S . Kentucky isolates were resistant to ciprofloxacin. Of these, 49 S . Kentucky isolates showed MIC values in the range of 3 to >256 μg/ml. Twenty-nine (41.43%) Salmonella isolates were co-resistant to ciprofloxacin and cefotaxime by disk-diffusion test. The tet A gene was detected in all Salmonella isolates and the bla TEM was the most predominant (25.37%) β-lactam gene. Multiple virulence genes such as sip A, mgt C, s op E1, s tn, sop B, fli C, spv C and gip A were also detected. High resistance to quinolone, cephalosporin and tetracycline antibiotics in nontyphoidal Salmonella isolates of poultry origin points towards their extra-label use in poultry farms. Highlights: Salmonella Kentucky as the predominant serotype in retail chicken shops. All Salmonella isolates (100%) are multidrug resistant. 41.43% Salmonella isolates co-resistant to ciprofloxacin and cefotaxime. High ciprofloxacin and levofloxacin MICs. bla TEM (25.37%) is the most predominant β-lactam gene detected.