DNA‐Mediated Proximity‐Based Assembly Circuit for Actuation of Biochemical Reactions. Issue 40 (10th September 2018)

• Record Type: Journal Article
• Authors: Oh, Sung Won; Pereira, Adriana; Zhang, Ting; Li, Tianran; Lane, Ariel; Fu, Jinglin
• Is Part Of: Angewandte Chemie international edition. Volume 57:Issue 40(2018)
• Page Start: 13086
• Page End: 13090
• Publication Date: 2018-09-10
• Subjects: biosensors -- DNA nanotechnology -- DNA assembly -- enzyme actuation -- logic gates; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201806749
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Smart nanodevices that integrate molecular recognition and signal production hold great promise for the point‐of‐care (POC) diagnostic applications. Herein, the development of a DNA‐mediated proximity assembly of biochemical reactions, which was capable of sensing various bio‐targets and reporting easy‐to‐read signals is reported. The circuit was composed of a DNA hairpin‐locked catalytic cofactor with inhibited activity. Specific molecular inputs can trigger a conformational switch of the DNA locks through the mechanisms of toehold displacement and aptamer switching, exposing an active cofactor. The subsequent assembly of an enzyme/cofactor pair actuated a reaction to produce colorimetric or fluorescence signals for detecting target molecules. The developed system could be potentially applied to smart biosensing in molecular diagnostics and POC tests. Abstract : DNA directed detection : A robust, smart DNA nanodevice capable of sensing various bio‐targets and reporting an easy‐to‐read signal was designed. A DNA logic‐gated structure was used to mediate the proximity‐based assembly of an enzyme/cofactor system that can detect target molecules by catalyzing the production of colorimetric or fluorescence signals.