DNA‐Mediated Proximity‐Based Assembly Circuit for Actuation of Biochemical Reactions. Issue 40 (10th September 2018) - Oh, Sung Won
Abstract: Smart nanodevices that integrate molecular recognition and signal production hold great promise for the point‐of‐care (POC) diagnostic applications. Herein, the development of a DNA‐mediated proximity assembly of biochemical reactions, which was capable of sensing various bio‐targets and reporting easy‐to‐read signals is reported. The circuit was composed of a DNA hairpin‐locked catalytic cofactor with inhibited activity. Specific molecular inputs can trigger a conformational switch of the DNA locks through the mechanisms of toehold displacement and aptamer switching, exposing an active cofactor. The subsequent assembly of an enzyme/cofactor pair actuated a reaction to produce colorimetric or fluorescence signals for detecting target molecules. The developed system could be potentially applied to smart biosensing in molecular diagnostics and POC tests. Abstract : DNA directed detection : A robust, smart DNA nanodevice capable of sensing various bio‐targets and reporting an easy‐to‐read signal was designed. A DNA logic‐gated structure was used to mediate the proximity‐based assembly of an enzyme/cofactor system that can detect target molecules by catalyzing the production of colorimetric or fluorescence signals.