Effect of D614G Spike Variant on Immunoglobulin G, M, or A Spike Seroassay Performance. (1st December 2020)

• Record Type: Journal Article
• Authors: Klumpp-Thomas, Carleen; Kalish, Heather; Hicks, Jennifer; Mehalko, Jennifer; Drew, Matthew; Memoli, Matthew J; Hall, Matthew D; Esposito, Dominic; Sadtler, Kaitlyn
• Is Part Of: Journal of infectious diseases. Volume 223:Number 5(2021)
• Page Start: 802
• Page End: 804
• Publication Date: 2020-12-01
• Subjects: SARS-CoV-2 -- coronavirus -- spike protein -- D614G -- pandemic -- COVID-19; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiaa743
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: Emergence of a new spike protein variant (D614G) with increased infectivity has prompted many to analyze its role in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. There is concern regarding whether an individual exposed to one variant of a virus will have cross-reactive memory to the second variant. Accordingly, we analyzed the serologic reactivity of both variants, and we found that antibodies from 88 donors from a high-incidence population reacted toward both the original spike and the D614 spike variant. These data suggest that patients who are exposed to either variant have cross-responsive humoral immunity. This represents an important finding both for SARS-CoV-2 disease biology and for therapeutics. Abstract : As the D614G spike variant emerged as the main severe acute respiratory syndrome coronavirus 2 variant in the United States, concerns arose regarding possible differences in antibody binding and serologic assay performance. We show that antibodies cross-react with both variants.