Influence of enhanced electrogenicity on anodic biofilm and bioelectricity production by a novel microbial consortium. (May 2021)

• Record Type: Journal Article
• Authors: Ajunwa, Obinna Markraphael; Odeniyi, Olubusola Ayoola; Garuba, Emmanuel Oluwaseun; Marsili, Enrico; Onilude, Abiodun Anthony
• Is Part Of: Process biochemistry. Volume 104(2021)
• Page Start: 27
• Page End: 38
• Publication Date: 2021-05
• Subjects: Anodic biofilm -- Bioelectrochemistry -- Exoelectrogenicity -- Pyocyanin -- Riboflavin; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2021.01.003
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Pseudomonads, Bacillus sp., Enterobacter sp. and Pichia sp. made up the consortium. Electrode pretreatment and metabolic enhancers improved consortium electrogenicity. Improved biofilm production was achieved using a metabolically enhanced consortium. Metabolic enhancement is a promising technique in bioelectricity generation. Abstract: Biofilms, flavins and pyocyanin have been identified as metabolites for species-specific electrogenicity. In this work, open circuit voltages (OCV) >500 mV earlier produced by electrogens in glucose-based media was used in confirming electrogens. Biofilms of test electrogens were grown on anodes of microbial fuel cells (MFCs) fed with glucose-based media. Electrogens used were anodophilic Enterobacter aerogenes 102, flavinogenic Bacillus sp. 101, Pichia kudriavzevii 103 and four pyocyanogenic Pseudomonads. Treatments with inhibitors (norspermidine, dichloro-diaminobenzene, and Raloxifene) negatively affected their electrogenicity. To enhance electrogenicity, increased anodic-biofilm was achieved by anode pretreatments with ultraviolet radiation and hydrogen peroxide, while pyocyanin-induced electrogenicity was enhanced by treatment with sophorolipds. Improved flavin yield and electrogenicity production was achieved by treatment with dimethyl-diaminobenzene. Bioelectricity production using metabolically-enhanced electrogens was carried out in a modified MFC and optimum yields were achieved using a consortium of all electrogens exposed to metabolic enhancements. Measurements of electrochemical potential showed improved electrogenicity in metabolically-enhanced consortium compared to the unenhanced consortium. Using eight MFCs in series, OCV value of 5000 mV was achieved, while parallel connections yielded increased current up to 6000 mA/m 2 . Electron microscopy showed well-developed biofilms formed by metabolically-enhanced consortium. EPS from consortium biofilm were also characterized using fourier transform-infrared (FT-IR) spectroscopy.