Emerging applications of site-directed spin labeling electron paramagnetic resonance (SDSL-EPR) to study food protein structure, dynamics, and interaction. (March 2021)

• Record Type: Journal Article
• Authors: Li, Hui; Pan, Yanxiong; Yang, Zhongyu; Rao, Jiajia; Chen, Bingcan
• Is Part Of: Trends in food science & technology. Volume 109(2021)
• Page Start: 37
• Page End: 50
• Publication Date: 2021-03
• Subjects: Food proteins -- Functionality -- Protein structure and dynamics -- Protein‒phenolic interaction -- Site directed spin-labeling (SDSL) -- Electron paramagnetic resonance (EPR); Food industry and trade -- Periodicals; Food -- Biotechnology -- Periodicals; 664.005
• DOI: 10.1016/j.tifs.2021.01.022
• Languages: English
• ISSNs: 0924-2244

Abstract

Abstract: Background: Specific interests in improving the functionality of food proteins as well as in protein‒food constituent interactions have resulted in a tremendous number of scientific papers. However, the fundamental mechanisms on protein structures and dynamics in different environments and conditions remain unknown, which hampers structure-based design of food proteins with predictable properties and desired functionalities. The technical barriers in probing structural basis of food protein functionality is one of the major barriers for such situation. Scope and approach: The aim of this review article is to delineate the ability of site directed spin-labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy to unravel food protein structure and dynamics in atomic levels. The review highlights the importance of food protein structures and dynamics in terms of their functionality in food systems. Detailed applications of SDSL-EPR in deciphering structural and dynamics information of biological proteins are provided. A few key areas that SDSL-EPR can help improve the understanding of the fundamental relationship between structure and functionality of food proteins are proposed. Lastly, the limitations of SDSL-EPR in food systems and the solutions are outlined. Key findings and conclusions: SDSL-EPR offers exceptional advantages to study food protein structure, dynamics, and interactions. The adoption of SDSL-EPR may open up a new horizon on understanding food protein structure and dynamic at an atomic level. Unlike the current trial-and-error methods, the information gained by SDSL-EPR could help food scientist rational design food systems with desirable functionalities based upon the ability to predict how protein structure affects functionality. Graphical abstract: Image 1 Highlights: SDSL-EPR reveals biological protein structures and dynamics in atomic levels. SDSL-EPR describes the relationship of lipase conformational dynamics and activity. SDSL-EPR provides time-resolved investigation on the dynamics of immobilized enzyme. The fundamental of protein‒phenolics interactions could be unveiled by SDSL-EPR. SDSL labels of a serial of recombinant proteins and site-direct mutagenesis.