A novel method for the simultaneous determination of esterified 2-/3-MCPD and glycidol in foods by GC-MS/MS. (May 2021)

• Record Type: Journal Article
• Authors: Zheng, Xiaoling; Fu, Wusheng; Zheng, Kuicheng; Gao, Bo; Lin, Lishan; Liu, Wenjing; Lin, Zhong; Fang, Qinmei
• Is Part Of: Food control. Volume 123(2021)
• Publication Date: 2021-05
• Subjects: 3-Monochloro-1 -- 2-propanediol ester -- 2-Monochloro-1 -- 3-propanediol ester -- Glycidyl ester -- Heptafluorobutyrylimidazole -- GC-MS/MS; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2020.107766
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Several critical improvements for simultaneous determination of 2- and 3-monochloropropanediol esters (2-MCPDE, 3-MCPDE) and glycidyl esters (GE) were established in this study. GE were converted into 3-monobromopropanediol monoesters (3-MBPDE) through bromination, then, 3-MBPDE, 2-MCPDE and 3-MCPDE were converted into their free forms through acid catalysis. The three target analytes were purified by matrix solid phase dispersion extraction and then derivatized with heptafluorobutyrylimidazole (HFBI). The derivatives were detected by GC-MS/MS, and key parameters including NaBr concentration, bromination time and temperature, and transesterification time were optimized. Finally the new procedure was fully validated. The limits of detection for 3-MCPDE, 2-MCPDE and GE in vegetable oil were 2.0, 5.0, and 10 μg/kg, respectively. The average recoveries ranged from 85.4% to 110% with relative standard deviation（RSD）of 1.3%–7.4% when the spiked levels varied from 0.05 to 1.0 mg/kg. The trueness of the method was also successfully and consistently evaluated by the proficiency test in FAPAS. Highlights: The combination of 3-MBPD and HFBI was first reported for quantification of GE. Analysis based on GC–MS/MS without a PTV injector. The trueness was evaluated by FAPAS proficiency test.