Estrogenic and antiandrogenic activities of methoxyflavans isolated from the fruit of Mauritia Flexuosa (Moriche Palm). Issue 1 (19th December 2020)

• Record Type: Journal Article
• Authors: Miyasaka, Kenchi; Takeda, Shogo; Nakamura, Seikou; Matsuda, Hisashi; Shimoda, Hiroshi
• Is Part Of: Journal of food biochemistry. Volume 45:Issue 1(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-12-19
• Subjects: 5α‐reductase -- estrogen -- Mauritiaflexuosa -- MCF‐7 -- methoxyflavan -- moriche palm -- prostate; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Biochemistry -- Periodicals; 664.024
• DOI: 10.1111/jfbc.13583
• Languages: English
• ISSNs: 0145-8884

Abstract

Abstract: Moriche palm is consumed as both a fresh fruit and processed food in Peru and Brazil. Although its fruit contains phytoestrogens, the active compounds have not yet been identified. Therefore, we purified moriche palm extract (MPE) and identified compounds exhibiting estrogenic and antiandrogenic activities. Estrogenic activity was assessed by the estrogen‐dependent growth of MCF‐7 cells and increases in uterine weights in mice. Antiandrogenic activity was evaluated by 5α‐reductase inhibitory activity and prostate‐specific antigen (PSA) expression in LNCaP cells. In vivo antiestrogenic activity was also assessed based on testosterone‐induced prostate growth in castrated mice. Four methoxyflavans were isolated from MPE and all, except for 7, 4′‐dihydroxy‐5‐methoxyflavan, promoted MCF‐7 cell growth, indicating estrogenic activity. Uterine and ovary weights increased in mice orally administered MPE (400 mg/kg) for 2 weeks. Regarding antiandrogenic activity, among the four methoxyflavans isolated, 6, 7, 4'‐trihydroxy‐5‐methoxyflavan (1 µg/ml) suppressed the mRNA and protein expression of PSA in LNCaP cells. Furthermore, prostate growth was suppressed in mice orally administered MPE (200 mg/kg) for 2 weeks. All methoxyflavans inhibited 5α‐reductase activity with IC50 less than 10 µg/ml. Collectively, the present results demonstrated that orally administered MPE exhibited estrogenic and antiandrogenic activities. Methoxyflavans, particularly 6, 7, 4′‐trihydroxy‐5‐methoxyflavan, appear to be the active compounds for these activities. Practical applications: The fruit of Mauritia flexuosa (moriche palm) has been used for beverages and processed foods. Although it is said to contain phytoestrogens, the active compounds have not yet been identified. In this study, we isolated and identified methoxyflavans exhibiting estrogenic and antiandrogenic activities. Among them, 6, 7, 4′‐trihydroxy‐5‐methoxyflavan appeared to be the most effective compounds for these activities. Abstract : Methoxyflavans in moriche palm fruit extract (MPE) exhibited estrogenic activity in MCF‐7 cells. MPE exhibited both estrogenic and antiandrogenic activities in mice. Suppression of prostate specific antigen expression and 5α‐reductase activity by methoxyflavans are involved in anti‐androgenic activity of MPE