Profiling protein expression in Klebsiella pneumoniae with a carbohydrate-based covalent probe. (15th January 2021)

• Record Type: Journal Article
• Authors: Metier, Camille C.; Peng, Jiaming; Xu, Yong; Wootton, Hayley; Riesi, Victoire; Lynham, Steven; Zadi, Sirine; Turner, Carrie; Wand, Matthew E.; Mark Sutton, J.; Wagner, Gerd K.
• Is Part Of: Bioorganic & medicinal chemistry. Volume 30(2021)
• Publication Date: 2021-01-15
• Subjects: Covalent probe -- Carbohydrate -- Klebsiella -- Bacterial profiling -- Antimicrobial resistance; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; Biochemistry -- Periodicals; Chemistry, Clinical -- Periodicals; Chemistry, Organic -- Periodicals; Chimie bio-organique -- Périodiques; Chimie pharmaceutique -- Périodiques; 615.19
• DOI: 10.1016/j.bmc.2020.115900
• Languages: English
• ISSNs: 0968-0896

Abstract

Graphical abstract: Highlights: A covalent probe based on d -glucosamine for the generation of bacterial signatures. A distinct signature was obtained for a carbapenem-resistant K. pneumoniae strain. Targets include low-abundance proteins not readily detectable by protein staining. Putative targets include several carbohydrate-recognising proteins. Abstract: We report the application of a covalent probe based on a d -glucosamine scaffold for the profiling of the bacterial pathogen Klebsiella pneumoniae . Incubation of K. pneumoniae lysates with the probe followed by electrophoretic separation and in-gel fluorescence detection allowed the generation of strain-specific signatures and the differentiation of a carbapenem-resistant strain. The labelling profile of the probe was independent of its anomeric configuration and included several low-abundance proteins not readily detectable by conventional protein staining. Initial target identification experiments by mass spectrometry suggest that target proteins include several carbohydrate-recognising proteins, which indicates that the sugar scaffold may have a role for target recognition.