Profiling protein expression in Klebsiella pneumoniae with a carbohydrate-based covalent probe. (15th January 2021) - Metier, Camille C.
Graphical abstract: Highlights: A covalent probe based on d -glucosamine for the generation of bacterial signatures. A distinct signature was obtained for a carbapenem-resistant K. pneumoniae strain. Targets include low-abundance proteins not readily detectable by protein staining. Putative targets include several carbohydrate-recognising proteins. Abstract: We report the application of a covalent probe based on a d -glucosamine scaffold for the profiling of the bacterial pathogen Klebsiella pneumoniae . Incubation of K. pneumoniae lysates with the probe followed by electrophoretic separation and in-gel fluorescence detection allowed the generation of strain-specific signatures and the differentiation of a carbapenem-resistant strain. The labelling profile of the probe was independent of its anomeric configuration and included several low-abundance proteins not readily detectable by conventional protein staining. Initial target identification experiments by mass spectrometry suggest that target proteins include several carbohydrate-recognising proteins, which indicates that the sugar scaffold may have a role for target recognition.