Comparative transcriptome analysis reveals the key regulatory genes for higher alcohol formation by yeast at different α-amino nitrogen concentrations. (May 2021)

• Record Type: Journal Article
• Authors: Wang, Ya-Ping; Sun, Zhong-Guan; Zhang, Cui-Ying; Zhang, Qiao-Zhen; Guo, Xue-Wu; Xiao, Dong-Guang
• Is Part Of: Food microbiology. Volume 95(2021)
• Publication Date: 2021-05
• Subjects: Saccharomyces cerevisiae, higher alcohol -- Transcriptome -- α-Amino nitrogen -- Alcoholic fermentation; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2020.103713
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: Higher alcohols are important flavor substance in alcoholic beverages. The content of α-amino nitrogen (α-AN) in the fermentation system affects the formation of higher alcohols by Saccharomyces cerevisiae . In this study, the effect of α-AN concentration on the higher alcohol productivity of yeast was explored, and the mechanism of this effect was investigated through metabolite and transcription sequence analyses. We screened 12 most likely genes and constructed the recombinant strain to evaluate the effect of each gene on high alcohol formation. Results showed that the AGP1, GDH1, and THR6 genes were important regulators of higher alcohol metabolism in S. cerevisiae . This study provided knowledge about the metabolic pathways of higher alcohols and gave an important reference for the breeding of S. cerevisiae with low-yield higher alcohols to deal with the fermentation system with different α-AN concentrations in the brewing industry. Highlights: Lower or higher α-amino nitrogen content leads to the production of higher alcohols. α-Amino nitrogen influences the metabolic networks of higher alcohols differently. Several metabolic pathways are the key factors affecting higher alcohol production. AGP1, GDH1, and THR6 genes are important regulators of higher alcohol metabolism.