Development of molecular markers to distinguish between morphologically similar edible plants and poisonous plants using a real‐time PCR assay. (11th September 2020)

• Record Type: Journal Article
• Authors: Kim, Geum Sol; Oh, Su Hong; Jang, Cheol Seong
• Is Part Of: Journal of the science of food and agriculture. Volume 101:Number 3(2021)
• Page Start: 1030
• Page End: 1037
• Publication Date: 2020-09-11
• Subjects: artificial gastric juice -- molecular markers -- poisonous plants -- qPCR -- SNPs; Food -- Periodicals; Agriculture -- Periodicals; 664
• DOI: 10.1002/jsfa.10711
• Languages: English
• ISSNs: 0022-5142

Abstract

Abstract: BACKGROUND: As a result of similar appearances between edible and poisonous plants, 42 patients have ingested poisonous plants from 2013 to 2017 in Korea. We have developed species‐specific primer sets of three of edible and poisonous plants sets ( Ligularia fischeri & Caltha palustris, Artemisia annua & Ambrosia artemisiifolia and Hemerocallis fulva & Veratrum maackii ) for distinguishing both plants using a real‐time polymerase chain reaction assay. RESULTS: The efficiencies of the developed primer sets ranged from 87.8% to 102.0%. The developed primer sets have significant correlation coefficient values between the Ct values and the log DNA concentration for their target species ( r 2 > 0.99). The cut‐off lines as the crossing point values of the limit of quantitation of the target species were determined, and all non‐target species were amplified later than the cut‐off cycles. Then, the effectiveness of the developed primer sets was evaluated using commercial food products and digested samples with simulated gastric juice. CONCLUSION: All of the developed species‐specific primer sets were able to detect target DNA successfully in commercial food products and the digested samples. Therefore, the developed species‐specific primer sets in the present study would be useful tools for distinguishing between poisonous plants and edible plants. © 2020 Society of Chemical Industry