A Ganoderma atrum polysaccharide alleviated DSS-induced ulcerative colitis by protecting the apoptosis/autophagy-regulated physical barrier and the DC-related immune barrier. Issue 12 (21st November 2020)

• Record Type: Journal Article
• Authors: Zheng, Bing; Ying, Mengxi; Xie, Jianhua; Chen, Yi; Wang, Yudan; Ding, Xiaomeng; Hong, Jiaqian; Liao, Wang; Yu, Qiang
• Is Part Of: Food & function. Volume 11:Issue 12(2020)
• Page Start: 10690
• Page End: 10699
• Publication Date: 2020-11-21
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/d0fo02260h
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : DSS destroyed the intestinal mucosa, while a Ganoderma atrum polysaccharide repaired the intestinal epithelial physical barrier and the DC-related immune barrier. Abstract : Polysaccharides are one of the main active substances in Ganoderma atrum ( G. atrum ). The purpose of this study was to explore the protective effect of a G. atrum polysaccharide (PSG-1) on DSS-induced colitis and the underlying mechanism. The results showed that PSG-1 could maintain the integrity of the intestinal structure by promoting the expression of goblet cells and levels of tight junction proteins in the colon of DSS-induced colitis mice. Furthermore, PSG-1 relieved the inhibition of Bcl-2 and the overexpression of caspase-3 and caspase-9 caused by DSS. Simultaneously, PSG-1 restored the expression of Atg5, Atg7 and beclin-1 and inhibited the p-akt and p-mTOR levels, suggesting that PSG-1 promoted autophagy via the Akt/mTOR pathway. Moreover, PSG-1 inhibited the content of DCs in the colon and modulated the expression of IL-10 in DCs. In conclusion, PSG-1 alleviated DSS-induced ulcerative colitis by protecting the apoptosis/autophagy-regulated physical barrier and the DC-related immune barrier.