Green recovery of Se-rich protein and antioxidant peptides from Cardamine Violifolia: Composition and bioactivity. (December 2020)

• Record Type: Journal Article
• Authors: Wu, Mailing; Zhu, Zhenzhou; Li, Shuyi; Cai, Jie; Cong, Xin; Yu, Tian; Yang, Wei; He, Jingren; Cheng, Shuiyuan
• Is Part Of: Food bioscience. Volume 38(2020)
• Publication Date: 2020-12
• Subjects: Cardamine violifolia -- Ultrasound-assisted extraction -- Se-rich protein -- Hydrolysate -- Antioxidant activity; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2020.100743
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: Cardamine violifolia (CV) is an important raw material for selenoprotein production. In this study, the protein of CV was extracted using conventional extraction (CE), ultrasound assisted extraction (UAE), pulsed electric pretreatment followed by CE (PEF + CE), and pulsed electric pretreatment followed by UAE (PEF + UAE), respectively. The result indicated that the yields of protein varied with extraction temperatures from 30 to 70 °C, while the highest yield was obtained at 40 °C for all these four methods. UAE showed the most effective extraction with a higher purity of protein powder (77%) and total selenium content (9097.33 ± 35.66 mg/kg). By application of response surface methodology, the protein yield was enhanced to 57.35 ± 0.92% at ultrasonic duration of 27 min, extraction temperature of 37 °C, and ultrasonic energy density of 8.125 W/mL. The obtained protein was further hydrolyzed by alcalase (37 °C, pH 10 for 5 h) and separated through ultrafiltration and size exclusion chromatography. Due to gel permeation chromatography-multi angle laser light scattering (GPC-MALLS) analysis, it was found that the CV protein hydrolysates were rich in dipeptides and tripeptides indicating promising antioxidant activities. Therefore, this study will contribute to the fabrication of selenium-rich proteins and antioxidant peptides as functional food ingredients. Graphical abstract: Image 1 Highlights: Protein from Cardamine Violifolia was extracted in comparison of four different methods. The Se-rich protein was obtained from ultrasound-assisted extraction after RSM analysis. Oligopeptides were separated from Se-rich protein hydrolysates with antioxidant activities.