Effects of covalent modification with epigallocatechin-3-gallate on oleosin structure and ability to stabilize artificial oil body emulsions. (30th March 2021)

• Record Type: Journal Article
• Authors: Sun, Yufan; Zhang, Shuang; Xie, Fengying; Zhong, Mingming; Jiang, Lianzhou; Qi, Baokun; Li, Yang
• Is Part Of: Food chemistry. Volume 341(2021)Part 2
• Publication Date: 2021-03-30
• Subjects: Artificial oil body -- Emulsion -- Oleosin -- Thermal stability -- Covalent modification; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.128272
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Oleosin (OL) was covalently conjugated to epigallocatechin-3-gallate (EGCG). OL and OL-EGCG conjugates were used to stabilize artificial oil body (AOB) emulsions. The suitability of AOB emulsions for the controlled release of curcumin was examined. An OL-EGCG/phosphatidylcholine complex was found to be a good AOB emulsion stabilizer. Highly stable emulsions with good encapsulation efficiency were realized. Abstract: The purpose of this study was to enhance the stability and functional properties of artificial oil body (AOB) emulsions. Herein, we covalently conjugated oleosin (OL) and epigallocatechin-3-gallate (EGCG) under alkaline conditions to obtain OL-EGCG conjugates. The results revealed that the structural characteristics of OL are improved by covalent binding to EGCG, with the OL-EGCG yield maximized at an EGCG concentration of 150 μM. We prepared AOB emulsions using native OL, the OL-EGCG conjugates, phosphatidylcholine (PC), and soybean oil for embedding curcumin. The results show that the protein components and phospholipids are bound in the AOB emulsion by hydrogen bonding and hydrophobic interactions. The covalent OL-EGCG/PC-stabilized emulsions exhibited more uniform droplet distributions, stronger thermal stabilities, and higher curcumin retentions than the other samples. These results indicated that the OL-EGCG/PC complexes are potential stabilizers for AOB emulsions and provided fresh insight into preparing highly stable emulsion embedding systems with good encapsulation efficiencies.