Synergistic antioxidant effects of phenolic acids and carotenes on H2O2-induced H9c2 cells: Role of cell membrane transporters. (30th March 2021)

• Record Type: Journal Article
• Authors: Pan, Yao; Deng, Ze-Yuan; Chen, Xuan; Zhang, Bing; Fan, Yawei; Li, Hongyan
• Is Part Of: Food chemistry. Volume 341(2021)Part 2
• Publication Date: 2021-03-30
• Subjects: BC β-Carotene -- CA Caffeic acid -- CD36 cluster of differentiation 36 -- CI Combination index -- DCFH-DA 2′, 7′-Dichlorofluorescein diacetate (DCFH-DA) -- DMSO Dimethyl sulfoxide -- EZT Ezetimibe -- FBS Fetal bovine serum -- GCLC Glutamate—cysteine ligase catalytic subunit -- HO-1 Heme oxygenase-1 -- H2O2 Hydrogen peroxide -- Keap1 Kelch-like ECH-associated protein 1 -- LY Lycopene -- MTT 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide -- Nrf2 Nuclear factor erythroid 2-related factor 2 -- NQO1 NAD(P)H dehydrogenase quinone 1 -- PA p-coumaric acid -- ROS reactive oxygen species -- SR Synergistic rate -- SR-BI Scavenger receptor class B, type I -- THF tetrahydrofuran -- TCM Traditional Chinese medicine; Synergistic effects -- Cell membrane transporters -- Carotenes -- Phenolic acids; β-Carotene (PubChem CID: 5280489) -- Caffeic acid (PubChem CID: 689043) -- p-Coumaric acid (PubChem CID: 637542) -- Lycopene (PubChem CID: 446925) -- Dimethyl sulfoxide (PubChem CID: 679) -- Tetrahydrofuran (PubChem CID: 8028) -- 2′, 7′-Dichlorofluorescein diacetate -- Diacetyldichlorofluorescein (PubChem CID: 104913) -- Hydrogen peroxide (PubChem CID: 784); Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.128000
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Explored the antioxidant interactions of different types of phytochemicals. Indicated that antioxidant interaction was affected by the ratios of phytochemicals. SR-BI/CD36-Nrf2/HO-1 may be one of the signaling pathways for antioxidant interaction among carotenes and phenolic acids. Intracellular antioxidant interaction among daily phytochemicals should consider their ratios and cellular uptake. Abstract: Phenolic acids (caffeic acid, p -coumaric acid, ) and carotenes (β-carotene, lycopene) were mixed in different ratios to investigate antioxidant interactions on H2 O2 -induced H9c2 cells with ezetimibe (inhibitor of carotenes membrane transporters). Cellular uptake of carotenes, expression of membrane transporters, reactive oxygen species (ROS), nuclear factor erythroid 2-related factor 2 (Nrf2), NAD(P)H dehydrogenase quinone1 (NQO1), heme oxygenase-1 (HO-1), glutamate-cysteine ligase catalytic subunit (GCLC) were analyzed. Results revealed that phenolic acids increased cellular uptake of carotenes and expression of their membrane transporters. Combination groups contained more phenolic acids showed synergistic effects. For example, β-carotene: caffeic acid = 1:2 significantly suppressed the intracellular ROS (+EZT, 66.34 ± 51.53%) and enhanced the accumulation of nucleus-Nrf2 (+EZT, 30.23 ± 5.30) compared to the groups contained more β-carotene (+EZT, ROS: 75.48 ± 2.55%, nucleus-Nrf2: 19.48 ± 4.22). This study provided an implication of functional foods formulation and demonstrated that antioxidant synergism may due to the up-regulation of carotenes membrane transporters by phenolic acids.