Antioxidant peptides from Mytilus Coruscus on H2O2-induced human umbilical vein endothelial cell stress. (December 2020)

• Record Type: Journal Article
• Authors: Zhang, Zhuangwei; Jiang, Shuoqi; Zeng, Yu; He, Kang; Luo, Yan; Yu, Fangmiao
• Is Part Of: Food bioscience. Volume 38(2020)
• Publication Date: 2020-12
• Subjects: Antioxidant -- Cytoprotection -- Human umbilical vein endothelial cells -- Mytilus coruscus -- Peptides; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2020.100762
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: The enzymatic protein hydrolysates of Mytilus coruscus (MCPH) were prepared with 5 proteases. The low molecular weight (MW) peptide fractions (<1 kDa, MCPH-T1) of trypsin hydrolysates were obtained using ultrafiltration. MCPH-T1 showed the most competitive DPPH, ABTS +, OH, and O2 − scavenging activities compared to other enzymatic hydrolysates or high MW fractions. Compared to glutathione, MCPH-T1 showed better ferric ion-reducing power and inhibited lipid peroxidation of linoleic acid. In H2 O2 -induced oxidative injured of human umbilical vein endothelial cells (HUVEC), MCPH-T1 pre-treatment improved cell viability and ameliorated the morphological damage through attenuating the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) production and restored the cellular antioxidant capacities. In addition, MCPH-T1 showed a cytoprotective effect on dysfunctional cell by regulating the expressions of apoptotic and NF-κB proteins and restored the Nrf2-driven antioxidant defense mechanism. These results indicated that MCPH-T1 may be potential food ingredients for managing oxidative stress-related endothelial dysfunction or related cardiovascular diseases. Graphical abstract: Image 1 Highlights: Mytilus coruscus protein hydrolysates were prepared with 5 proteases. MCPH-T1 (<1 kDa) trypsin hydrolysates showed better antioxidant activities. MCPH-T1 restored the antioxidant defense system and reduced ROS and MDA. MCPH-T1 regulated Caspase/NF-κB apoptosis and Nrf2 oxidative stress pathways.