Melatonin retards senescence via regulation of the electron leakage of postharvest white mushroom (Agaricus bisporus). (15th March 2021)

• Record Type: Journal Article
• Authors: Li, Ling; Kitazawa, Hiroaki; Zhang, Xinhua; Zhang, Liming; Sun, Yang; Wang, Xiangyou; Liu, Zhanli; Guo, Yanyin; Yu, Shaoxuan
• Is Part Of: Food chemistry. Volume 340(2021)
• Publication Date: 2021-03-15
• Subjects: ATP adenosine triphosphate -- ETC electron transport chain -- ROS reactive oxygen species -- PPO polyphenol oxidase -- SOD superoxide dismutase -- CAT catalase -- APX ascorbate peroxidase -- AOX alternative oxidase -- AsA-GSH cycle ascorbic acid glutathione cycle -- Cyt c/a cytochrome c/a -- RH relative humidity -- TPA texture profile analysis -- GR glutathione reductase -- RNS reactive nitrogen species; Agaricus bisporus -- Melatonin -- Senescence -- Electron transport chain -- Electron leakage; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.127833
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Electron leakage, the direct source of ROS and RNS, is prevented by melatonin. Melatonin has proven to retard the aging of mushroom by inhibiting electron leak. Melatonin alleviates the electron leakage through regulating the respiratory states. Melatonin prominently dampens the electron leakage by regulating the ETC and Cyt c. Abstract: Currently, melatonin ( N-acetyl -5-methoxytrytamine) is recognized as a potential scavenger of free radicals. In this study, the effect of exogenous melatonin at various concentrations (0.05, 0.1, and 0.2 mM) on the texture, sensory qualities, and electron leakage in white mushrooms was evaluated at 3 ± 1 °C. It was observed that mushrooms treated with 0.1 mM melatonin were of good quality and their electron leakage was dramatically dampened. The results showed that 0.1 mM melatonin retained a higher adenosine triphosphate level and also prevented the release of cytochrome c into the cytoplasm. More significantly, it prominently inhibited electron leakage by increasing the activities of complexes I and III by the upregulation of AbNdufB9 and AbRIP1 . It also regulated respiratory states in mushrooms; delayed the decline of respiratory state 3; enhanced respiratory state 4; boosted the oxidative phosphorylation and efficiency of mitochondria; and ultimately retarded the senescence of the white mushrooms.