Delayed utilization of some fast-fermenting soluble dietary fibers by human gut microbiota when presented in a mixture. (May 2017)

• Record Type: Journal Article
• Authors: Tuncil, Yunus E.; Nakatsu, Cindy H.; Kazem, Ahmad E.; Arioglu-Tuncil, Seda; Reuhs, Bradley; Martens, Eric C.; Hamaker, Bruce R.
• Is Part Of: Journal of functional foods. Volume 32(2017)
• Page Start: 347
• Page End: 357
• Publication Date: 2017-05
• Subjects: AX arabinoxylan (sorghum) -- CS chondroitin sulfate -- GM galactomannan -- XG xyloglucan -- PGA polygalacturonic acid -- Mixture containing equal amounts (weight basis) of AX, CS, GM, XG, and PGA; Colonic microbiota -- Dietary fiber -- Short chain fatty acid -- Fecal fermentation -- 16S rRNA gene sequencing; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.03.001
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Soluble dietary fibers generally are fast fermenting in the proximal colon. Delayed in vitro fermentation of soluble fibers was achieved when in a mixture. Certain fibers increased same genera in microbiota of different donors. SCFAs response to fiber fermentations varied by donor. Abstract: Delivering fibers more distally could be important to prevent or treat colonic diseases such as cancer and ulcerative colitis. Here, we hypothesized that fermentation of fast-fermenting soluble fibers by the colonic microbiota is delayed when they are presented in a mixture due to hierarchical utilization of fibers. A series of in vitro fermentation studies was performed using fecal microbiota obtained from three healthy donors using single dietary fibers [arabinoxylan, chondroitin sulfate (CS), galactomannan (GM), polygalacturonic acid (PGA), xyloglucan (XG)] and a mixture containing an equal amount of each. Substrate disappearance analysis, as measured by GC–MS, revealed that CS, PGA, and XG utilization was delayed when present in the mixture. 16S rRNA sequencing showed certain fibers consistently increased specific genera in the microbiota of all donor groups. Mixing different types of fermentable dietary fibers might be a logical strategy for delivering fibers into more distal regions of the colon.