Beneficial effects of anthocyanins from red cabbage (Brassica oleracea L. var. capitata L.) administration to prevent irinotecan-induced mucositis. (May 2017)

• Record Type: Journal Article
• Authors: Tong, Tao; Niu, Yun-Hui; Yue, Yuan; Wu, Shuang-chan; Ding, Hong
• Is Part Of: Journal of functional foods. Volume 32(2017)
• Page Start: 9
• Page End: 17
• Publication Date: 2017-05
• Subjects: AB/PAS alcian blue and periodic acid-Schiff's staining -- ACs Anthocyanins -- CIGT chemotherapy-induced gut toxicity -- HPLC high-performance liquid chromatography -- GSH glutathione -- LPS lipopolysaccharides -- MPO myeloperoxidase -- MTT 3-(4, 5-dimethyl thiazol-2-yl)-5-diphenyl tetrazolium bromide -- ROS reactive oxygen species -- TBARS thiobarbituric acid reactive substances -- TER Transepithelial electrical resistance -- TJ tight junction -- UGT1A uridine-diphospho-glucuronosyl transferase; Anthocyanins -- Autophagy -- Irinotecan -- mTOR -- Mucositis -- Red cabbage; Irinotecan (PubChem CID: 60838) -- 7-Ethyl-10-hydroxycamptothecin (PubChem CID: 104842) -- Loperamide (PubChem CID: 3955) -- Cyanidin-3-glucoside (PubChem CID: 92131208); Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.01.051
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Anthocyanins (ACs) from red cabbage inhibited Irinotecan-induced mucositis in mice. ACs protected the integrity of epithelial cells in vivo and in vitro . ACs regulated autophagy through suppressing the mTOR pathway. Abstract: Intestinal mucositis is a common side effect of Irinotecan (CPT-11). Anthocyanins (ACs) from red cabbage against CPT-11-induced mucositis were studied. Mice were given CPT-11 (75 mg/kg, i.p. for 4 days) and treated with ACs (50, 100 mg/kg, i.g. for 7 days). On day 8, diarrhea and leukocyte count in blood were assessed. Samples of intestine were obtained for morphometric analysis, myeloperoxidase (MPO), TBARS and GSH assay. The effects of ACs on cytoprotection and intestinal permeability were studied in Caco-2 cells. ACs effectively reversed the signs of intestinal injury, including MPO activity, length of ileum and colon, leukopenia, intestinal architecture, and quantity of ileum mucus. In vitro, pretreatment with ACs enhanced transepithelial electrical resistance (TER) and the expression of ZO-1. Furthermore, the protective effects of ACs were associated to modulate autophagy through mTOR pathway. In conclusion, ACs from red cabbage are candidate for supplementary therapy of CPT-11-induced mucositis.