MicroRNA-212 targets SIRT2 to influence lipogenesis in bovine mammary epithelial cell line. Issue 2 (16th May 2020)

• Record Type: Journal Article
• Authors: Lu, Xubin; Xia, Hailei; Jiang, Jingyi; Xu, Xin; Li, Mingxun; Chen, Zhi; Sun, Yujia; Zhang, Huimin; Yang, Zhangping
• Is Part Of: Journal of dairy research. Volume 87:Issue 2(2020)
• Page Start: 232
• Page End: 238
• Publication Date: 2020-05-16
• Subjects: Lipogenesis, -- miR-212, -- miR-375, -- miR-655, -- SIRT2; Dairying -- Periodicals; Dairying -- Research -- Periodicals; 636.214205
• DOI: 10.1017/S0022029920000229
• Languages: English
• ISSNs: 0022-0299

Abstract

Abstract: In this research paper we filter and verify miRNAs which may target silent information regulator homolog 2 ( SIRT2 ) gene and then describe the mechanism whereby miRNA-212 might regulate lipogenic genes in mammary epithelial cell lines via targeting SIRT2 . Bioinformatics analysis revealed that the bovine SIRT2 gene is regulated by three miRNAs: miR-212, miR-375 and miR-655 . The three miRNAs were verified and screened by qRT-PCR, western blot, and luciferase multiplex verification techniques and only miR-212 was shown to have a targeting relationship with SIRT2. The results of co-transfecting miR-212 and silencing RNA (siRNA) showed that by targeting SIRT2, miR-212 can regulate the expression of fatty acid synthetase ( FASN ) and sterol regulatory element binding factor 1 ( SREBP1 ) but not peroxisome proliferator-activated receptor gamma ( PPAR γ). Measurement of triglyceride (TAG) content showed that miR-212 increased the fat content of mammary epithelial cell lines. The study indicates that miR-212 could target and inhibit the expression of the SIRT2 gene to promote lipogenesis in mammary epithelial cell lines.