Effect of flaxseed oil on muscle protein loss and carbohydrate oxidation impairment in a pig model after lipopolysaccharide challenge. Issue 8 (28th April 2020)

• Record Type: Journal Article
• Authors: Kang, Ping; Wang, Yang; Li, Xiangen; Wan, Zhicheng; Wang, Xiuying; Zhu, Huiling; Wang, Chunwei; Zhao, Shengjun; Chen, Huifu; Liu, Yulan
• Is Part Of: British journal of nutrition. Volume 123:Issue 8(2020)
• Page Start: 859
• Page End: 869
• Publication Date: 2020-04-28
• Subjects: Protein kinase B/Forkhead box O signalling pathways, -- Carbohydrate oxidation impairment, -- Flaxseed oil, -- Muscle atrophy, -- Toll-like receptor 4/nucleotide-binding oligomerisation domain protein signalling pathway; Nutrition -- Periodicals; 572.4
• DOI: 10.1017/S0007114519002393
• Languages: English
• ISSNs: 0007-1145

Abstract

Abstract: Flaxseed oil is rich in α-linolenic acid (ALA), which is the metabolic precursor of EPA and DHA. The present study investigated the effect of flaxseed oil supplementation on lipopolysaccharide (LPS)-induced muscle atrophy and carbohydrate oxidation impairment in a piglet model. Twenty-four weaned pigs were used in a 2 × 2 factorial experiment including dietary treatment (5 % maize oil v . 5 % flaxseed oil) and LPS challenge (saline v . LPS). On day 21 of treatment, the pigs were injected intraperitoneally with 100 μg/kg body weight LPS or sterile saline. At 4 h after injection, blood, gastrocnemius muscle and longissimus dorsi muscle were collected. Flaxseed oil supplementation increased ALA, EPA, total n -3 PUFA contents, protein:DNA ratio and pyruvate dehydrogenase complex quantity in muscles ( P < 0·05). In addition, flaxseed oil reduced mRNA expression of toll-like receptor (TLR) 4 and nucleotide-binding oligomerisation domain protein (NOD) 2 and their downstream signalling molecules in muscles and decreased plasma concentrations of TNF- α, IL-6 and IL-8, and mRNA expression of TNF- α, IL-1 β and IL-6 ( P < 0·05). Moreover, flaxseed oil inclusion increased the ratios of phosphorylated protein kinase B (Akt) 1:total Akt1 and phosphorylated Forkhead box O (FOXO) 1:total FOXO1 and reduced mRNA expression of FOXO1, muscle RING finger (MuRF) 1 and pyruvate dehydrogenase kinase 4 in muscles ( P < 0·05). These results suggest that flaxseed oil might have a positive effect on alleviating muscle protein loss and carbohydrates oxidation impairment induced by LPS challenge through regulation of the TLR4/NOD and Akt/FOXO signalling pathways.