Survival of Salmonella enterica and shifts in the culturable mesophilic aerobic bacterial community as impacted by tomato wash water particulate size and chlorine treatment. (September 2020)

• Record Type: Journal Article
• Authors: Van Haute, Sam; Luo, Yaguang; Bolten, Samantha; Gu, Ganyu; Nou, Xiangwu; Millner, Patricia
• Is Part Of: Food microbiology. Volume 90(2020)
• Publication Date: 2020-09
• Subjects: Tomatoes -- Salmonella -- Chlorine wash -- Particulate-association -- Cross-contamination -- Packinghouse; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2020.103470
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: Particulates of harvest debris are common in tomato packinghouse dump tanks, but their role in food safety is unclear. In this study we investigated the survival of Salmonella enterica and the shifts in relative abundance of culturable mesophilic aerobic bacteria (cMAB) as impacted by particulate size and interaction with chlorine treatment. Particulates suspended in grape tomato wash water spanned a wide size range, but the largest contribution came from particles of 3–20 μm. Filtration of wash water through 330 μm, applied after 100 mg/L free chlorine (FC) wash, reduced surviving cMAB by 98%. The combination of filtration (at 330 μm or smaller pore sizes) and chlorinated wash also altered the cMAB community, with the survivors shifting toward Gram-positive and spore producers (in both lab-simulated and industrial conditions). When tomatoes and harvest debris inoculated with differentially tagged Salmonella were washed in 100 mg/L FC for 1 min followed by filtration, only cells originating from harvest debris survived, with 85 and 93% of the surviving cells associated with particulates larger than 330 and 63 μm, respectively. This suggests that particulates suspended in wash water can protect Salmonella cells from chlorine action, and serve as a vector for cross-contamination. Highlights: Debris particulates 3–20 μm in size in grape tomato wash water predominate. Filtering (≤330 μm) after washing (100 mg/L FC) reduces Salmonella survival 98%. Gram-positives, spore-formers dominate survivor microbiome of filtered wash water. Particulates in tomato wash water protect Salmonella from chlorine disinfection.