Bioassay guided fractionation of bioactive metabolite from Corynascus verrucosus inhabiting Croton bonplandianus Baill. (November 2020)

• Record Type: Journal Article
• Authors: Chandra Mohana, N.; Rakshith, D.; Yashavantha Rao, H.C.; Ramesha, K.P.; Nuthan, B.R.; Satish, S.
• Is Part Of: Process biochemistry. Volume 98(2020)
• Page Start: 106
• Page End: 112
• Publication Date: 2020-11
• Subjects: Mycoendophyte -- Corynascus verrucosus -- Bioactive metabolite -- TLC – bioautography; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.07.007
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: This work is the first report on incidence of Corynascus verrucosus as mycoendophyte. The bioactive moiety had significant activity against human pathogens. Bioassay fractionated bioactive emodin was identified by hyphenated spectroscopy. Abstract: Mycoendophytes have been a promising source of bioactive metabolites for new developments in pharmaceuticals and industrial relevance. During the screening of mycoendophytes diversity from C. bonplandianus, bioactive C. verrucosus CBF 22 was isolated from the leaf. The bioactive isolate was subjected to fermentation and the ethyl acetate fraction was evaluated for antimicrobial activity by disc diffusion and microbroth dilution methods, followed by cytotoxicity using MTT assay. The results revealed significant activity against Gram negative bacteria, Gram positive bacteria, and human pathogenic dermatophytic fungi, C. albicans . The TLC Bioautography-Guided approach employed for the detection of antimicrobial metabolite from bioactive fraction was depicted by a zone of inhibition. Antimicrobial screening of mycoendophytes C. bonplandianus could envision a unique potential niche for bioactive compounds of pharmaceutical importance. These findings suggest C. verrucosus as a potent candidate for producing natural bioactives, to combat multidrug-resistant pathogens. This work is the first report on the incidence of C. verrucosus as mycoendophyte and its bioactive potential of inhabiting C. bonplandianus Baill.