Targeting of ovarian cancer cell through functionalized gold nanoparticles by novel glypican-3- binding peptide as a ultrasound contrast agents. (November 2020)

• Record Type: Journal Article
• Authors: Dongying, Qu; Lan, Liao; Qian, Ding
• Is Part Of: Process biochemistry. Volume 98(2020)
• Page Start: 51
• Page End: 58
• Publication Date: 2020-11
• Subjects: Gold nanoparticles -- Glypican-3 protein -- Cell viability -- Cellular uptake image; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.07.019
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: A new GLY-3 peptide targeting model developed and fabricated to AuNPs (GPC3@AuNPs). The binding and uptake of both naked and GPC3@AuNPs were analyzed by CLSM in human HeLa cells. The results displayed that the GLY-3 functionalized AuNPs remarkably improved the ultrasound contrasts. Abstract: Gold nanoparticles (AuNPs) are widely studied nanomaterials for their potential employment in advanced biomedical applications, such as selective molecular imaging and targeted drug delivery. AuNPs are generally low cost and highly biocompatible, can be easily functionalized with a wide variety of functional ligands, and have been demonstrated to be effective in enhancing ultrasound contrast at clinical diagnostic frequencies. Therefore, AuNPs might be used as contrast agents in echographic imaging. In this work, we have developed a AuNPs -based system for the in vitro molecular imaging of ovarian carcinoma cells that express high levels of glypican-3 protein (GPC-3) on their surface. In this regard, a novel GPC-3 targeting peptide was designed and conjugated to fluorescent AuNPs nanoparticles. The physicochemical properties, acoustic behavior, and biocompatibility profile of the functionalized AuNPs were characterized. Then, the binding and uptake of both naked and functionalized AuNPs were analyzed by laser scanning confocal microscopy in human HeLa cells (ovarian carcinoma) cell line. The results obtained showed that GPC-3-functionalized fluorescent AuNPs significantly enhanced the ultrasound contrast and were effectively bound and taken up by HeLa cells without affecting their viability.