Preparation of a broad-spectrum anti-zearalenone and its primary analogues antibody and its application in an indirect competitive enzyme-linked immunosorbent assay. (1st May 2018)

• Record Type: Journal Article
• Authors: Dong, Guoliang; Pan, Yuanhu; Wang, Yulian; Ahmed, Saeed; Liu, Zhenli; Peng, Dapeng; Yuan, Zonghui
• Is Part Of: Food chemistry. Volume 247(2018)
• Page Start: 8
• Page End: 15
• Publication Date: 2018-05-01
• Subjects: Zearalenone -- Monoclonal antibody -- Indirect competitive enzyme-linked immunosorbent assay -- Feed -- Animal edible tissue; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2017.12.016
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Molecular modelling was used to hapten designing and antibody analyzing. A sensitive and broad-spectrum mAb against ZEN and its analogues was obtained. A mAb-based ic-ELISA was developed for ZEN and its analogues in various samples. Abstract: A broad-spectrum monoclonal antibody (mAb)-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed for rapidly screening zearalenone (ZEN) and its primary analogues in various samples using an easy sample preparation procedure. Primarily, a group-specific mAb, 6C2, was produced, which had IC50 values for ZEN, α-zearalenol, β-zearalenol, α-zearalanol, β-zearalanol and zearalanone of 114.0, 127.4, 290.4, 114.9, 205.6 and 257.1 ng L −1, respectively. The limit of detection and limit of quantitation of this method for ZEN and its five primary analogues in various matrix samples ranged from 114.2 to 812.3 ng L −1 and 237.1 to 1653.9 ng L −1, respectively. The recoveries of the above samples spiked with ZEN and its five primary analogues were in the range of 62.9–113.6%. The CVs were less than 13.2%. A good correlation (R 2 = 0.995) between the ic-ELISA results and the HPLC-MS/MS results for swine feeds supported the reliability of the developed ic-ELISA.