Recent advances in universal TA cloning methods for use in function studies. Issue 11 (22nd October 2016)

• Record Type: Journal Article
• Authors: Yao, Shuo; Hart, Darren J.; An, Yingfeng
• Is Part Of: Protein engineering, design & selection. Volume 29:Issue 11(2016)
• Page Start: 551
• Page End: 556
• Publication Date: 2016-10-22
• Subjects: directional cloning -- high-throughput cloning -- protein expression -- TA cloning -- T-vector; Protein engineering -- Periodicals; 660.63
• DOI: 10.1093/protein/gzw047
• Languages: English
• ISSNs: 1741-0126

Abstract

Abstract: As one of the simplest and most efficient cloning methods, T-vector-based TA cloning has been widely used for cloning of single genes and construction of DNA libraries. This approach is especially suitable for high-throughput cloning of diverse DNA fragments since inserts can be cloned without knowledge of their sequence; it is therefore an ideal tool for high-throughput analysis of protein structure and function. Although most of the currently available T-vectors can only be used for cloning purposes, some novel variants with improved functions have be developed. This review focuses on recent developments of universal TA cloning methods and T-vectors constructed for function studies.