Polyphenol-rich extract of Zhenjiang aromatic vinegar ameliorates high glucose-induced insulin resistance by regulating JNK-IRS-1 and PI3K/Akt signaling pathways. (15th January 2021)

• Record Type: Journal Article
• Authors: Xia, Ting; Duan, Wenhui; Zhang, Zhujun; Fang, Bin; Zhang, Bo; Xu, Bicheng; de la Cruz, Celia Bertha Vargas; El-Seedi, Hesham; Simal-Gandara, Jesus; Wang, Shaoyun; Wang, Min; Xiao, Jianbo
• Is Part Of: Food chemistry. Volume 335(2021)
• Publication Date: 2021-01-15
• Subjects: Insulin resistance -- JNK-IRS-1 -- PI3K/Akt -- Vinegar extract -- Polyphenols -- HepG2 cells; Akt protein kinase B -- G6Pase glucose-6-phosphatase -- GS glycogen synthase -- GSK3β glycogen synthase kinase 3β -- IR insulin resistance -- IRS-1 insulin receptor substrate-1 -- JNK c-Jun NH2 terminal kinase -- MAPK mitogen activated protein kinase -- p-Akt phosphorylated protein kinase B -- PEPCK phosphoenolpyruvate carboxykinase -- p-GS phosphorylated glycogen synthase -- p-GSK3β phosphorylated glycogen synthase kinase 3β -- PI3K phosphatidylinositol 3-kinase -- p-IRS-1 (Ser307) phosphorylated insulin receptor substrate-1 (Ser307) -- p-JNK phosphorylated c-Jun NH2 terminal kinase -- p-PI3K phosphorylated phosphatidylinositol 3-kinase -- ROS reactive oxygen species -- T2DM type 2 diabetes mellitus -- ZAV Zhenjiang aromatic vinegar -- ZAVE Zhenjiang aromatic vinegar extract; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.127513
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Zhenjiang aromatic vinegar extract increased the glucose uptake and consumption in cells. Zhenjiang aromatic vinegar extract enhanced glycogen synthesis and attenuated gluconeogenesis. Zhenjiang aromatic vinegar extract regulated JNK-IRS-1 and PI3K/Akt signaling pathways. Abstract: Zhenjiang aromatic vinegar is a famous traditional fermented cooking ingredient in China, with multiple nutritional and medicinal applications. Zhenjiang aromatic vinegar extract (100–400 μg/mL) is rich in polyphenols increased the glucose uptake and glucose consumption in high glucose-induced insulin resistant HepG2 (IR-HepG2) cells. Zhenjiang aromatic vinegar extract enhanced glycogen synthesis and attenuated gluconeogenesis by regulating key enzymes in IR-HepG2 cells. In addition, Zhenjiang aromatic vinegar extract ameliorated high glucose-induced IR by inhibiting phosphorylated insulin receptor substrate-1 (IRS-1) expression and activating phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway in IR-HepG2 cells. Moreover, Zhenjiang aromatic vinegar extract reduced reactive oxygen species generation and phosphorylated c-Jun NH2 terminal kinase (JNK) expression in IR-HepG2 cells. The attenuation of the high glucose is owned to the PI3K/Akt pathway activation, glycogen synthesis induction and gluconeogenesis suppression in IR-HepG2 cells.