A sensitive immunoassay based on fluorescence resonance energy transfer from up-converting nanoparticles and graphene oxide for one-step detection of imidacloprid. (15th January 2021)

• Record Type: Journal Article
• Authors: Guo, Yirong; Zou, Rubing; Si, Fangfang; Liang, Wenlong; Zhang, Tianyi; Chang, Yunyun; Qiao, Xvsheng; Zhao, Jinhao
• Is Part Of: Food chemistry. Volume 335(2021)
• Publication Date: 2021-01-15
• Subjects: Imidacloprid -- Fluorescence resonance energy transfer -- Graphene oxide -- Up-converting nanoparticles -- Immunoassay; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.127609
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: An efficient FRET immunoassay for imidacloprid was constructed by UCNPs and GO. The heterologous competitive format had better sensitivity than the homologous one. The method showed high sensitivity and good accuracy with the validation of UPLC-MS/MS. The method is suitable for rapid detection of imidacloprid in food and environmental samples. Abstract: In this study, a fluorescence resonance energy transfer (FRET) immunoassay based on graphene oxide (GO) and up-converting nanoparticles (UCNPs) was established for rapid detection of imidacloprid, a commonly-used insecticide. Under 980 nm near-infrared light excitation, emission of UCNPs at 542 nm can be absorbed by the energy acceptor GO. The carboxyl-functionalized GO and UCNPs were coupled with competitive antigen and antibody against imidacloprid. After optimization, the FRET immunoassay showed a wide detection range of 0.08–50 ng/mL to imidacloprid, with cross-reaction toward other three neonicotinoids including imidaclothiz (74.4%), thiacloprid (36.9%) and clothianidin (31.9%). The average recoveries of spiked water, Chinese cabbage, cucumber, honey and tea samples were 76.8%-101.8%. The accuracy and reliability of the FRET immunoassay were verified by UPLC-MS/MS with a good correlation (R 2 = 0.9816). In a summary, this study provides a sensitive and one-step method for monitoring imidacloprid residue in food and environmental samples within 1 h.