Prevalence, toxin-typing, and antimicrobial susceptibility of Clostridium perfringens from retail meats in Seoul, Korea. (August 2020)

• Record Type: Journal Article
• Authors: Jang, Yong-Seok; Kim, Dong-Hyeon; Bae, Dongryeoul; Kim, Se-Hyung; Kim, Hyunsook; Moon, Jin-San; Song, Kwang-Young; Chon, Jung-Whan; Seo, Kun-Ho
• Is Part Of: Anaerobe. Volume 64(2020)
• Publication Date: 2020-08
• Subjects: Clostridium perfringens -- Prevalence -- Retail meat -- Toxin-typing -- Antimicrobial resistance -- Multiple-drug resistance; Anaerobic infections -- Periodicals; Anaerobic bacteria -- Periodicals; Bacterial diseases -- Periodicals; Computer network resources; Anaerobic protozoa -- Periodicals; 579.3
• DOI: 10.1016/j.anaerobe.2020.102235
• Languages: English
• ISSNs: 1075-9964

Abstract

Abstract: Clostridium perfringens is a ubiquitous, Gram-positive, spore-forming bacterium. It can contaminate many types of retail meat products and cause food poisoning by producing enterotoxins in the small intestines of humans and domestic animals. We investigated the prevalence, toxin-encoding gene profile, and antimicrobial resistance of C. perfringens in beef, chicken, and pork meat purchased from retail markets in Seoul, Korea. C. perfringens was detected according to the International Organization for Standardization 7937, with some modifications, and confirmed using the Vitek 2 system. In total, 38 C. perfringens strains were isolated from 200 meat samples (38/200, 19%; thirty-three from chicken, and five from beef). Among the six toxins evaluated, including alpha, beta, epsilon, iota, enterotoxin (encoded in the cpe gene), and netB, only the cpa gene was detected in all isolates by polymerase chain reaction (PCR) amplification. The antimicrobial resistance of the isolates was evaluated using the agar dilution method and resistance to ampicillin (12/38, 31.6%), tetracycline (38/38, 100%), chloramphenicol (26/38, 68.4%), metronidazole (13/38, 34.2%), and imipenem (27/38, 71%) was observed. Interestingly, 30 of the 38 isolates (78.9%) were multiple-drug resistant, showing resistance to more than three different antimicrobial classes. Highlights: We investigated Clostridium perfringens in beef, chicken, and pork meat. All isolates were cpe -negative C. perfringnes type A. Thirty of 38 isolates (78.9%) were resistant to multiple drugs.